APAF1 Polyclonal Antibody | A0752_APAF1.pdf |
Catalog No: A0752 | Species: Rabbit | |
Gene ID: 317 | Isotype: IgG | |
Swiss Prot: O14727 | Purity: Affinity purification | |
Applications: WB IHC ICC | Cross-Reactivity: H M | |
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| 100ug | ＄300 | Ship in 5 business days | Adding to the cart | | |
| 200ug | ＄480 | Ship in 5 business days | Adding to the cart | | |
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Alt. Names
CED4; APAF-1;
Recommended Dilutions
WB: 1:500-1:2000
IHC: 1:50-1:100
ICC: 1:50-1:100
Products data
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Western blot analysis of extracts of HeLa cell line, using APAF1 antibody. | | |
Immunogen
A synthetic peptide of human APAF1
Storage
Store at -20oC or -80oC. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.