比普通DIG随机引物法标记效率高,采用CSPO化学发光检测灵敏度更高.一个kit可用于12次标记反应和24次检测反应组成;5×高效Dig标记复合物;未标记对照DNA,PBR328;DNA稀释液;抗-Dig-碱性磷酸酶连接物;CSPD;10×封闭液;Dig定量试纸条;Dig对照试纸条;NBT/BCIP储存液,-20℃保存
Catalog # Product name / Packsize
11585614910
(1585614) DIG-High Prime DNA Labeling and Detection Starter Kit II
1 kit (12 labeling reactions and 24 detection reactions)
Application
DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.
Product Description
Sensitivity and specificity: Standard assay uses 1 μg of linearized pBR328. It is usual to expect 0.8 μg labeled DNA after 1 h of labeling, and 2.3 μg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 μg of digested placenta DNA.
Contents
DIG-High Prime, 5x conc., 50 μl
DIG-labeled Control DNA, 20 μl, (5 μg/ml) pBR328 (linearized with Bam HI)
DNA Dilution Buffer, 3 x 1 ml
Anti-digoxigenin-AP Conjugate, 50 μl
CSPD, ready-to-use, 50 ml
Blocking Solution, 10x conc., 4 x 100 ml
DIG Easy Hyb Granules, 4 x 100 ml