Immunoassays have a long and successful history for protein quantification and the first publications on elisa date back almost 40 years. However, there are also cases where these classic approaches reach their limits.
How about your assays? Do you always find your analyte and can you reliably quantify it? Do you sometimes face matrix issues, and do you find samples simply outside the LOQ? How important is it for you to quantify beyond a simple “non-detectable“ when a clinical project is at stake?
Chimera´s Imperacer Assay System goes the extra step towards sensitivity and robustness. As proven in hundreds of clinical projects the proprietary PCR-amplification of the immuno-detection shifts the limits of detection and quantification by up to three orders of magnitude compared to conventional methods. And sometimes more important still; the AnySource concept circumvents matrix effects and gets you the more robust assay fort he real samples in preclinical and clinical settings.
Whether you employ the Imperacer services for assay development, validation, and sample analysis is your choice. The alternative is now entirely in your hands. With the Imperacer WorkStation and the Assay Development Kits or custom made kits for your assay you can run it all in your lab, even under GLP conditions.
Imperacer® Workstation
The Imperacer® Workstation bundle is designed for cost-effective Imperacer® bioanalysis (maximum throughput of 2000 data points / week). The package is designed to perfectly conduct Imperacer® assays in the daily laboratory routine and summarizes Chimera Biotec´s 10 year experience in Imperacer® assay development and routine bioanalysis.
The Imperacer® Workstation comprises the following components:
- Imperacer® Workstation - Hardware
- Imperacer® Workstation - Initial Equipment
- Imperacer® Workstation - Imperacer® assay development kit
- Imperacer® Workstation - PCR Maintenance Kit
- Imperacer® Workstation - Imperacer® Assay development training
Imperacer® Workstation - Hardware
- Imperacer® Reader: The core component of any Imperacer® workstation is an Imperacer®-enabled PCR Cycler.
- Imperacer® Washer: The Imperacer® Washer is a combination of speed, simplicity and performance and has diverse assay protocols and programs.
- Imperacer® enabled PC with Monitor, Keyboard and Mouse: The workstation comes equipped with a controlling PC for data collection and initial analysis.
- Table top lab equipment: To comply with the requirements of an ultra-sensitive Imperacer® immunoassay, the table top equipment comprises a Microplate Shaker, Thermomixer, Mini-centrifuge and Vortex.
- Imperacer® Singlechannel Pipette: This set of pipettes has proven to be a very useful tool for precise handling.
- Imperacer® Multichannel Pipette: These adjustable pipettes have proven to be very useful tools for precise sample transfer from standard cup formats to Imperacer® microplate formats.
Imperacer® Workstation - Initial Equipment
The Initial Equipment, composed by Chimera Biotec, is intended to ensure minimal sensitivity loss and limited reagent adhesion to plastic surfaces.
- Silikonated cups
- Pipette tips
- Imperacer sample preparation tubes
Imperacer® Workstation - Imperacer® assay development kit
Chimera offers Imperacer® assay developments kits in combination with a training by an Imperacer® specialist. The customer learns to develop his own ultra-sensitive assay from scratch by using biotinylated detection antibodies.
Imperacer® Workstation - PCR Maintenance Kit
The PCR maintenance kit is designed for first time initialization and optimal inspection of the Imperacer reader performance in planned intervals.
Imperacer® Workstation - Imperacer® Assay development training
On-site training enabling rapid and convenient technology transfer of Imperacer® into your laboratory routine