Kon,koff,KD,Label-freekineticsoncells,Measureprotein-smallmolecule,Measuresmallmolecules

sam^®X Top of the range

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Key points

Top of the range system
Optimized for label-free kinetics on cells
Highest Throughput – 8 sensors on two sensor chips
Ultimate flexibility – discrete sample delivery to a single sensor, or with fluidic switching access pairs or group of four channels
On-line sample loading

sam®X – Chips through windows and Chipholder
Chips viewed through the windows on sam^®X and sensor chips for sam^®X

Key applications:

Concentration measurements
K_on, k_off, K_D
Label-free kinetics on cells e.g. therapeutic monoclonal antibodies, bispecific antibodies
Measure protein-small molecule (<200 Da) and protein-protein interactions all on one platform
Measure protein conformational change on small molecule or allosteric inhibitor binding
Measure small molecules in high DMSO concentrations with no signal interference or correction
Measurements of crude samples with little or no purification

Which sam instrument is for me?

	sam^®X	sam^®5 GREEN	sam^®5 BLUE
Positioning	Addresses the user with highest demands to flexibility in method & assay development, throughput, automation and workflow standardization	Addresses the user focusing controlled accuracy and perpetuation of high quality demands in method development & routine assays	Addresses the user focusing high versatility in method & assay development, user friendliness and low running costs
Target Group	Pharma	Biotech	Academia
Sensor chips	2 chips	1 chips	1 chips
Number of sensor channels	8 (4 sensor channels per chip)	5	5
Sensor chip re-use	No Chips are disposable when chemistry is exhausted	No Chips are disposable when chemistry is exhausted	Yes User can strip chemistry to gold and re-coat
Fluidic path for sensor chip	Sensor channels are individually addressable through the fluidics or in combinations: 8 x 1, 4 x 2, 2 x 4, 1 x 8	Sequential across all 5 sensor channels of the chip	Sequential across all 5 sensor channels of the chip
Binding of different capture proteins on chip surface	On-line using fluidics to address individual sensor channels	Off-line using a coating device	Off-line using a coating device
Relative chip pricing (where 1 is lowest)	1	2	3
Fluidic cell replacement	Annual and needs a service engineer	Software prompt based on tracking chip usage, user can change	Recommended annual and user can change
Buffer switching	Automatic 2 buffer bottles	Manual 1 buffer bottle	Manual 1 buffer bottle
Key benefit	combines high precision & reliability with flexibility & automation	combines high precision & reliability with reproducibility & consistency	combines high precision & reliability with versatility & robustness

sam^®X

sam^®5 GREEN

sam^®5 BLUE

Positioning

Addresses the user with highest demands to flexibility in method & assay development, throughput, automation and workflow standardization

Addresses the user focusing controlled accuracy and perpetuation of high quality demands in method development & routine assays

Addresses the user focusing high versatility in method & assay development, user friendliness and low running costs

Target Group

Pharma

Biotech

Academia

Sensor chips

2 chips

1 chips

Number of sensor channels

8 (4 sensor channels per chip)

Sensor chip re-use

No
Chips are disposable when chemistry is exhausted

Yes
User can strip chemistry to gold and re-coat

Fluidic path for sensor chip

Sensor channels are individually addressable through the fluidics or in combinations: 8 x 1, 4 x 2, 2 x 4, 1 x 8

Sequential across all 5 sensor channels of the chip

Binding of different capture proteins on chip surface

On-line using fluidics to address individual sensor channels

Off-line using a coating device

Relative chip pricing (where 1 is lowest)

Fluidic cell replacement

Annual and needs a service engineer

Software prompt based on tracking chip usage, user can change

Recommended annual and user can change

Buffer switching

Automatic
2 buffer bottles

Manual
1 buffer bottle

Key benefit

combines high precision & reliability with
flexibility & automation

combines high precision & reliability with
reproducibility & consistency

combines high precision & reliability with
versatility & robustness

Kon,koff,KD,Label-freekin…

sam®X Top of the range

Key points

Key applications:

Which sam instrument is for me?

sam^®X Top of the range