实时无标记生物传感器,Real…

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实时无标记生物传感器,Real-time,label-freebiosensors
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  • 参考报价：电议 产地：德国 品牌：saw-instruments 型号：SAW‘s innovative 更新时间：2023/4/3

Real-time, label-free biosensors

  • Measures binding kinetics and affinity constants
  • Uses surface acoustic wave detection technology
  • Orthogonal approach to SPR, QCM-D and calorimetry
Learn more

sam®X bionsensor system without autosampler

Features of our Biosensors

  • Flow through system with fluidic switching Flow through system with
    fluidic switching
  • Chip-based binding Chip-based binding and measurement
  • sam®X and Autosampler Autosampler enables unattended throughput
  • our sensors are easy to use Easy to use

 

 

Technology

Technology

SAW Instruments is based on the application of its proprietary Surface Acoustic Wave (SAW) technology in biosensors for real-time, label free measurements of biomolecular interactions. The machines of SAW Instruments use a detection technology resembling a highly sensitive "weight detector". Binding processes are measured by real mass changes on the surface.

Basically, the sensor is a chip, whereby the surface is excited to oscillate acoustically. The acoustic waves are generated in its piezoelectric material and travers laterally. Response to changes in the surface-bound material and configuration is a modified oscillation with a proprietary method. This method and the high operation frequency significantly reduces distortion effects by the complexity of matrix, therefore purification steps might be ommited.

The phase of the wave is shifted on mass changes. Viscoelastic and conformational characteristics are indicated by a change in amplitude. Both effects can be differentiated and are detected independently to be used for interaction analysis (see figure c).

Need for next generation biosensor Feature of SAW Benefit of SAW
To work with whole viable cells, without fixing, to look at binding to native membrane proteins Suspension cells – load directly through the fluidics and capture on chips enabling
To work with small molecules/fragment libraries in varying sample concentrations of DMSO Adherent cells – grow on the chips
High S/N;
Eliminates DMSO from baseline
Aspect ratio of >150:1;

Generate accurate KD;
Only need to run reference sample once and not for every sample		 Performance
Ability to get additional structural information on conformational changes Amplitude parameter can monitor changes in active site structure (e.g. enzymes) on ligand binding enabling and time-saving as may not need to co-crystallize
Ability to work with blood, high serum and unpurified proteins Remove limitations of optical systems and less sample prep/purification Performance and time-saving
Ability to regenerate chip surfaces and low running costs For sam®5 BLUE, sensor chips can be stripped back down to base gold surface and recoated with new chemistries Economical
Larger penetration depth for detection Enables work with long strands of DNA and also binding to cells is possible Performance
Real-time kinetics Phase parameter measures real-time kon, koff, KD Equivalent to Response Units (RU) for SPR
Concentration Analysis An order of magnitude improvement over SPR Performance
Increased number of sensors 5 sensor channels on the sam®5 sensor chips;
8 (2 x 4) sensor channels on sam®X sensor chips		 Throughput and performance
Eliminate mass transport effects at low flow rates Large optimized chip surface area Throughput and performance
Eliminate the need for reference channels Proprietary 2-frequency mode Performance Accuracy Performance
Increased sensor channel surface area Can load more capture protein down on the chip surface Performance / Sensitivity
Low service and maintenance costs No optical components Economical
Does not require an expert to run Used by many teaching universities and can support multiple groups Simple
No compromise on sensitivity Parity with SPR (0.5pg/mm2)
Opportunities to increase using gold and nanoparticles		 Performance
Chip surfaces beyond Gold SiO2 future-proof & enabling

Which sam instrument is for me?

sam®X
  sam®X sam®5 GREEN sam®5 BLUE
Positioning Addresses the user with highest demands to flexibility in method & assay development, throughput, automation and workflow standardization Addresses the user focusing controlled accuracy and perpetuation of high quality demands in method development & routine assays Addresses the user focusing high versatility in method & assay development, user friendliness and low running costs
Target Group Pharma Biotech Academia
Sensor chips 2 chips 1 chips 1 chips
Number of sensor channels 8 (4 sensor channels per chip) 5 5
Sensor chip re-use No
Chips are disposable when chemistry is exhausted		 No
Chips are disposable when chemistry is exhausted		 Yes
User can strip chemistry to gold and re-coat
Fluidic path for sensor chip Sensor channels are individually addressable through the fluidics or in combinations: 8 x 1, 4 x 2, 2 x 4, 1 x 8 Sequential across all 5 sensor channels of the chip Sequential across all 5 sensor channels of the chip
Binding of different capture proteins on chip surface On-line using fluidics to address individual sensor channels Off-line using a coating device Off-line using a coating device
Relative chip pricing (where 1 is lowest) 1 2 3
Fluidic cell replacement Annual and needs a service engineer Software prompt based on tracking chip usage, user can change Recommended annual and user can change
Buffer switching Automatic
2 buffer bottles		 Manual
1 buffer bottle		 Manual
1 buffer bottle
Key benefit combines high precision & reliability with
flexibility & automation		 combines high precision & reliability with
reproducibility & consistency		 combines high precision & reliability with
versatility & robustness

 sam®X

Top of the range

sam®X Zoom

Key points

  • Top of the range system
  • Optimized for label-free kinetics on cells
  • Highest Throughput – 8 sensors on two sensor chips
  • Ultimate flexibility – discrete sample delivery to a single sensor, or with fluidic switching access pairs or group of four channels
  • On-line sample loading

sam®X – Chips through windows and Chipholder
Chips viewed through the windows on sam®X and sensor chips for sam®X

Key applications:

  • Concentration measurements
  • Kon, koff, KD
  • Label-free kinetics on cells e.g. therapeutic monoclonal antibodies, bispecific antibodies
  • Measure protein-small molecule (<200 Da) and protein-protein interactions all on one platform
  • Measure protein conformational change on small molecule or allosteric inhibitor binding
  • Measure small molecules in high DMSO concentrations with no signal interference or correction
  • Measurements of crude samples with little or no purification

 

sam®5 GREEN   Assures quality

sam®5 GREEN Zoom

Key points

  • Assures quality
  • 5 channels – five sensor-channels on one sensor chip
  • Work-flow tracking – chip-based online workflow tracking to enable consistent application monitoring
  • Reproducibility – application monitored pre-coated sensor chips to cope with the highest quality demands for methods development and routine assays
  • Disposable chips
  • Contiguous flow through of sample across all 5 sensor positions
  • Off-line loading of different binders using coating device

sam®5 GREEN – chipholder sam®5 GREEN
Chipholder sam®5 GREEN

Key applications

  • Concentration measurements
  • Kon, koff, KD
  • Protein-protein and small-molecule (<200 Da) interactions on a single platform
  • Measure protein conformational change on small molecule or allosteric inhibitor binding
  • Measure small molecules in high DMSO concentrations with no signal interference or correction
  • Crude samples with little or no purification

sam®5 BLUE   Ideal for Academics

sam®5 BLUE Zoom

Key points

  • The “original” sam
  • Ideal for academics
  • 5 channels – five sensor-channels on one sensor chip
  • Re-coatable chips – high versatility in method and assay development due to re-coatable sensor chips combined with low running costs
  • Availability of plain gold chips for making own coatings
  • Easy to use – robust and easy to handle system, simple to clean and maintain, optimal for basic research and teaching
  • Contiguous flow through of sample across all 5 sensor positions
  • Off-line loading of different binders using coating device

chipholder sam®5 BLUE Fluidic Cell
Chipholder sam®5 BLUE and Fluidic Cell

Key applications:

  • Concentration measurements
  • Kon, koff, KD
  • Measure protein-small molecule (<200 Da) and protein-protein interactions all on one platform
  • Measure protein conformational change on small molecule or allosteric inhibitor binding
  • Measure small molecules in high DMSO concentrations with no signal interference or correction
  • Use crude samples with little or no purification
  • On-line biosensor to LC-MS connectivity

Sensor Chips and Chemistries

Fluidic cell

Chips are available in a range of pre-coated chemistries. They are sold in packs of 3, and we also supply kits with a range of chemistries included.

For sam®5 GREEN and sam®5 BLUE, an off-line coating device is provided for attaching different discrete capture molecules onto the different sensor positions of the chip. Once the proteins are spotted, they are allowed to attach, then the chip is washed and dried before insertion into the biosensor.

For the sam®X, the capture proteins can be immobilised on-line due to the novel plumbing which enables discrete targetting of a sample to a specific position on the chip. This means chip loading can be monitored and measured in real-time.

Chip/chemistry coatings  
Plain gold sam®5 BLUE only
COOH SAM All models
OH SAM All models
CH3 SAM All models
NH2 SAM All models
Carboxymethyl Dextran (long chain) All models
Carboxymethyl Dextran (short chain) All models
Streptavidin Dextran All models
Protein A/G All models

sam®5 BLUE
The coating tool containing a chip on the chip holder ready for off-line immobilisation of proteins.

Software

A simple three-part software package supports all the sam® models. It controls the complete measurement, performs the desired injections and enables analysis of the resulting curves. The system comes with 1 permanent licence for the well-established ORIGIN software for data analysis.

Logo: SequenceMaster®

Define the process flow

Experimental Setup
SequenceMaster® supports the assembly of specific customer programs for the execution of a complete injection series by the autosampler. The SequenceMaster® provides an easy to use "Drag & Drop" interface to create sam® Biosensor programs.

Logo: SenseMaster®

Run the measurement

Data Acquisition
The measuring and controlling software SensMaster® provides a graphical interface, which controls the execution of the experiments. With SensMaster®, measuring parameters are set and data acquistion can then be tracked in real-time. Both the phase and amplitude parameters can be tracked simultaneously.

Logo: FitMaster®

Get the information

Data Evaluation
FitMaster® is used for the analysis. Measurement data is exported from SensMaster® into ORIGIN to be analyzed and processed automatically with FitMaster®. Depending on the experiment, the data can be analyzed with defined standard binding models

KD determinations in minutes
FitMaster® provides a set of different KD calculation models enabling the user to determine KD automatically. Calculation results can be easily obtained in minutes from the measurement data. For both binding and KD, customized models can be developed and implemented. Data can also be exported to other analysis alternative packages if a more complex fit or model is required.

A simple three-part software package supports the sam®5 system. It controls the complete measurement, performs the desired injections and enables analysis of the resulting curves.

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