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Cell surface carbohydrates act as specific recognition molecules for intercellular adhesion among certain cells.
Glycosphingolipids represent a group of cell surface glycoconjugates that are easily separated and analyzed by thin layer chromatography. The cell adhesion chamber was specifically designed to permit the assay of cell adhesion directly on glycosphingolipids separated on thin layer chromatograms.
Cells are centrifuged directly on the TLC plate to form immediate close contact with chromatographed glycosphingolipids. The position of the bound cells corresponds to the Rf value of the glycosphingolipid ligand which may be used for purification and structural characterization of the functional carbohydrate sequence.
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