35mm培养皿循环流动室,Circ…

35mm培养皿循环流动室,CircularFlowChamber(for35mmtissueculturedishes)
产品简介
详细介绍
  • 参考报价:电议 产地:美国 品牌:glycotech 型号:31-001 更新时间:2023/4/3

Circular Flow Chamber (for 35mm tissue culture dishes)

 

31-001

Circular Flow Chamber Kit ($675.00)

 

Technical Reference: Improvements to Parallel Plate Flow Chambers to Reduce Reagents and Cellular Requirements, Brown and Larson, BMC Immunology 2001, 2:9

 

Flow Chamber Kit Includes:

Two flow decks
 

 

(cast acrylic with different inlet/outlet designs)

8 silicon rubber gaskets

 

(two of each size)
Flow Width = 0.25 cm, Thickness = 0.005 in
Flow Width = 0.25 cm, Thickness = 0.010 in
Flow Width = 0.50 cm, Thickness = 0.010 in
Flow Width = 1.00 cm, Thickness = 0.010 in

24 polypropylene tubing connectors

(six of each)
Threaded 1/16 in. straight and L-connectors
Male and Female taper luer to 1/16 in. connectors

Silastic? laboratory tubing (1/16 in. ID x 10 ft.)

 

Replacement Gaskets and Coverslips for the Circular Flow Chamber
31-002 Four Gaskets (Flow Width = 0.25 cm, Thickness = 0.005 in) 询价
 
31-003 Four Gaskets (Flow Width = 0.25 cm, Thickness = 0.010 in) 询价
 
31-004 Four Gaskets (Flow Width = 0.50 cm, Thickness = 0.010 in) 询价
 
31-005 Four Gaskets (Flow Width = 1.00 cm, Thickness = 0.010 in) 询价
 
31-008 Circular Glass Coverslips (pack of 30, 35 mm, #2) 询价
 
Parallel-Plate Flow Chamber
描 述:
平行板流动小室(Parallel-Plate Flow Chamber)是体外研究细胞力学最常用的模型之一,用来模拟体内各种细胞处于生理环境中,暴露在动态液体流作用下所受到的流体剪应力。体外细胞的代谢反应与所受到的壁面剪应力密切相关。
典型的平行板流动小室由三个部分组成:聚碳酸酯分液室,硅胶垫圈和玻璃盖玻片。分液室构成流动小室的一侧,包括进液口,出液口和真空槽。垫圈的厚度决定流程长度。盖玻片构成流动小室的另一侧,其上覆盖细胞外基质蛋白(ECM),血管细胞或其他待研究的生物材料。真空形成一个密封环境将三个部件紧密连接在一起,同时也确保通道高度的一致性。
优 势:
1.平行板流动小室能够产生生理学范畴内的壁面剪应力:0.01-30 dyn/cm2。通过注射泵以可控的运动力将液体(如抗凝的全血或者细胞悬液)注入小室并流进固定化基质的方法产生剪应力。
2.装置设计,组装和操作简单,方便;
3.可以在设定的时间-周期内研究稳定剪应力对细胞的影响;也可以随实验需求调节流速和剪应力;
细胞可在流动条件下生长,能够在显微镜下随时观察生长状态;或者利用视频显微镜实时监控细胞状态;
应 用:
1.模拟体内血液流动产生的血流剪切力,研究不同剪应力下白细胞和内皮细胞之间的动态粘附作用或白细胞受体-配体间的相互作用等;
2.利用平行板流动小室产生剪应力模拟肿瘤细胞的体内生长环境,从而研究肿瘤细胞的增殖,吸附和转移机制。
3.细胞趋化实验中作为药物测试体系,基于白细胞-内皮细胞粘附过程作为新药物的运输体系;
产品介绍:
Glycotech公司提供两种平行板流动小室:Rectangular Flow Chamber(矩形流动小室)和Circular Flow Chamber(圆形流动小室),含有平行流动小室装置需要的基本组成部件。客户根据自身实验需求选择合适的产品。
(1).Rectangular Flow Chamber(用于标准显微载玻片)
Rectangular Flow Chamber KitCat#:31-010)
组成成分:
1流动板 (灌注的聚丙烯酸材质)
4个 硅橡胶垫圈(每种尺寸各2个)
Flow Width = 1.00 cm, Thickness = 0.005 in
Flow Width = 1.00 cm, Thickness = 0.010 in
24个 聚丙烯管接头(每种尺寸各6个)
Threaded 1/16 in. straight and L-connectors
Male and Female taper luer to 1/16 in. connectors
硅橡胶实验软管(1/16 in. ID x 10 ft.)
 
(2).Circular Flow Chamber(用于35mm组织培养皿)
Circular Flow Chamber Kit(Cat#:31-001)
组成成分:
2流动板 (灌注的聚丙烯酸材质,带有不同的进出口设计)
8硅橡胶垫圈(每种尺寸各2个)
Flow Width = 0.25 cm, Thickness = 0.005 in
Flow Width = 0.25 cm, Thickness = 0.010 in
Flow Width = 0.50 cm, Thickness = 0.010 in
Flow Width = 1.00 cm, Thickness = 0.010 in
24个聚丙烯管接头(每种尺寸各6个)
Threaded 1/16 in. straight and L-connectors
Male and Female taper luer to 1/16 in. connectors
硅橡胶实验软管(1/16 in. ID x 10 ft.)
产品订购:
品牌
名称
货号
规格
价格
Glycotech
Rectangular Flow Chamber Kit
31-010
1 kit
询价
Glycotech
Circular Flow Chamber Kit
31-001
1 kit
询价
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世联博研北京技有限公司 免费电话:400-650-8506 E-mailslby800@163.com
应用文献:
1.Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation, Rockville, Maryland 20850
2. FLOW CHAMBER FOR STUDYING CELL ATTACHMENT TO OPAQUE SUBSTRATES. EDUARDO ALBERTO REYES.
3. Physiologic Stress-Mediated Signaling in the Endothelium. Cynthia A.
4. Covalent Immobilization of P-Selectin Enhances Cell Rolling. Seungpyo Hong.
5. Improvements to parallel plate flow chambers to reduce reagent and cellular requirements. David C Brown.
6. Analysis of Physiologic E-Selectin-Mediated Leukocyte Rolling on Microvascular Endothelium.Georg Wiese.

 

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