product specifications

 

expression bioarrays

 

CodeLink Human Whole Genome Bioarray

 

CodeLink™ Human Whole Genome Bioarray has 54,841 probes representing up to 57,347 unique transcripts. The transcript sequences were selected from the NCBI UniGene build #165, the RefSeq database (January 5, 2004 release), and the dbEST database (January 8, 2004 release). In addition to the discovery probe sequences, each bioarray contains positive and negative bacterial control probes for monitoring array performance. All oligonucleotide probes are 30 bases long.

Probe sets	Probes/gene	Redundancy	Dispense count
Number of human probes	One probe/transcript x 54,841 unique sequences	1	54,841 probes
Positive bacterial control probes	5 probes/gene × 6 bacterial targets	12	360 probes
Negative bacterial control probes	32 negative control probes	12	384 probes
TOTAL			55,585 probes

Performance specifications

 

The following data are based on results from the final product verification studies conducted using a series of bioarrays and targets generated from human tissue.

Performance parameter	Performance requirement	Verified assay performance
Specificity	Discrimination > 2 base mismatches	Discrimination > 2 base mismatches
Sensitivity	1:600,000 mass ratio (bacterial spike)*	1:2,048,000 mass ratio (bacterial spike)*; 1:1,024,000 mass ratio with mRNA spiking at total RNA level**
Dynamic range	Linear signal response over two orders of magnitude	Linear signal response over three orders of magnitude; 0.05 pM – 50 pM
Signal reproducibility (total CV)	< 20% total median CV	19.6% total median CV; 8.2% median CV for all spots excluding spots below noise
Minimum detectable fold change	95% within two‐fold change for the same tissue	97.6% within two‐fold change for the same tissue for all good spots
Input sample	0.1 µg – 2 µg total RNA	0.1 – 2.0 µg total RNA***

* mass ratio spiking of cRNA control spike into cRNA target

** the lowest level tested in verification experiments with mRNA spiking

*** generates sufficient amplified material for 1‐8 replicates depending on tissue and starting amount

Storage and handling conditions

 

Upon receipt, store CodeLink™ Human Whole Genome Bioarrays with desiccant at room temperature.

Safety precautions

 

 

Standard laboratory safety procedures should be followed when using this product. Safety glasses, a lab coat, and appropriate gloves should be worn at all times when in the laboratory.

© Applied Microarrays, Inc. 2007—All rights reserved.

All goods and services are sold subject to the terms and conditions of sale of Applied Microarrays, Inc. which supplies them. A copy of these terms and conditions is available on request.

CodeLink is a trademark of General Electric Company.

Notice to Purchaser – Important License Information

The purchase of this nucleic acid array includes a limited license for the purchaser to use the nucleic acid array for its own internal research purposes only. The purchaser may not transfer data derived from the use of this nucleic acid array to third parties, and may not resell this nucleic acid array.

The purchaser may not license or otherwise transfer a database of gene expression data containing five hundred data points or more (where a single data point is data relating to the level of expression of one gene in one sample) created through the use of this nucleic acid array to a third party or otherwise use this nucleic acid array to contribute to the creation of such a database for licensing or otherwise transferring to a third party. The purchaser may not use this nucleic acid array for the identification of antisense reagents or the empirical design of probes or sets of probes for using or making nucleic acid arrays.

The purchase of this nucleic acid array does not include or carry any right or license for the purchaser to use such

nucleic acid array in the provision of services to third parties, including the transfer of data derived from the use of nucleic acid arrays. If you wish to use nucleic acid arrays for commercial purposes, including the provision of services, please contact your local Applied Microarrays, Inc. representative.

Additional terms and conditions are applicable to purchases of this nucleic acid array. These additional terms and conditions can be found at www.appliedmicroarrays.com and/or in accompanying product documentation.

This nucleic acid array and its use are covered by the following patents owned by Oxford Gene Technology Limited or Oxford Gene Technology IP Limited: US 6,054,270, Eur. 0 373 203, Jap. 3,393,528 and 3,386,391, US 6,150,095 and 6,307,039 and pending patents.