These improved films for classic thermal cycling applications consist of a 50 µm polyester film with a 41 µm layer of acrylic adhesive. Compared to the original ThermalSeal® films (below), ThermalSeal 2™ films offer better adhesion, reduced evaporation, longer end tabs for easier handling, and less tendency to tear during removal from the plate. Each sealing film measures 79.4 x 139.7 mm and offers sufficient sealing area for all PCR plates. Length between the perforations with end tabs removed is 125.1 mm. (Not recommended for raised-rim plates; see ThermalSeal A™ films below.) ThermalSeal 2 sealing films are not pierceable. For applications where piercing with pipet tips or robotic probes is required for product recovery, see AlumaSeal® sealing films. For real-time PCR applications where maximum optical clarity is required, see ThermalSeal RT and RTS sealing films below.

• heat resistant, recommended for temperatures from -40 °C to +120 °C
• better adhesion; reduced evaporation
• longer end tabs for easier handling
• does not tear during removal from the plate
• easier to apply than aluminum foils, no tendency to roll back
• certified DNase-, RNase-, and nucleic-acid-free

A 50 µm heat-resistant polypropylene sealing film with a 25 µm layer of acrylic adhesive designed for thermal cycling applications. (Not recommended for PCR plates with narrow or irregular sealing surfaces: ThermalSeal 2™, AlumaSeal®, ThermalSeal A™, or ThermalSeal RT™ series films are better in such instances.) Each sealing film measures 79.4 x 135.1 mm and offers sufficient sealing area for all PCR plates. Length between the perforations with end tabs removed is 123.1 mm. ThermalSeal sealing films are not pierceable. For applications where piercing with pipet tips or robotic probes is required for product recovery, see AlumaSeal® sealing films. For real-time PCR applications where maximum optical clarity is required, see ThermalSeal RT and RT2RR sealing films below.

• heat resistant, recommended for temperatures from
  -40 °C to +120 °C
• easier to apply than aluminum foils, no tendence to roll back
• certified DNase-, RNase-, and nucleic-acid-free

ThermalSeal^® MiniStripsTM sealing films are identical to ThermalSeal sealing films (above), but designed for sealing only one or two 8-well rows at a time on PCR plates or strip tubes. They are now supplied in convenient sheets of four. Each sealing film strip is 19.0 x 97.3 mm, including two removable positioning tabs. The long dimension with tabs removed is 76.2 mm. Each package of 200 contains 50 sheets of four sealing film strips.

• two tabs on each strip for easy application and removal
• perforated tabs can be removed or sealed around plate edge
• heat resistant, recommended for temperatures from
  -40 °C to +120 °C
• certified DNase-, RNase-, and nucleic-acid-free

ThermalSeal ATM sealing films are an advanced version of Excel Scientific's classic ThermalSeal^® sealing films for PCR. ThermalSeal A films consist of 50 µm thick clear polypropylene with a thicker 50 µm layer of a stronger adhesive formulated for improved protection against evaporation and leakage during thermal cycling. Intended for non-real-time PCR applications, ThermalSeal A films are designed to fit inside the edge of raised-rim PCR plates and provide more secure sealing of all wells because the center of the film does not extend over the plate rim. Two end tabs allow for easy positioning of the film on the plate but are perforated for easy removal if necessary. Each film measures 77.8 x 135.5 mm overall. Length between the perforations wth end tabs removed is 118.1 mm. Each package of ThermalSeal A films includes one sealing paddle for ensuring a firm seal, also compatible with raised-rim plates.

• stronger and thicker 50 µm adhesive layer for secure seal
• heat resistant, recommended for temperatures from
  -40 °C to +120 °C
• can be used with raised-rim PCR plates and standard plates
• two end tabs, perforated for easy removal
• certified DNase-, RNase-, and nucleic-acid-free

ThermalSeal RTTM sealing films combine an optically transparent polyester sealing film with a strong, ultra-smooth, non-absorbing, non-fluorescing medical-grade adhesive for superior performance in real-time PCR applications. In place of the customary paper backing, ThermalSeal RT sealing films have a plastic liner which is easily removed before use and contributes smoothness and extreme optical clarity to the adhesive. Dimensions are 79.4 by 142.9 mm for sealing PCR plates. Length between the perforations with end tabs removed is 121.9 mm.

• "brilliant" ultra-high optical clarity
• heat resistant, recommended for temperatures from
  -40 °C to +120 °C ***
• certified DNase-, RNase-, and nucleic-acid-free

ThermalSeal RT2RRTM 50 µm polyester sealing sealing films for real-time PCR are sized to fit within the edges of raised-rim 96-well plates. The same consistent ultra-high optical clarity as Excel's ThermalSeal RTTM sealing films (above) makes possible more reproducible, reliable, and consistent DNA amplification measurements. An inert, strong, temperature-resistant adhesive assures reliable sealing around each well. Two end tabs assist in positioning the sealing film. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with sealing films that overlap the plate rim. Recommended for specific DNA sequence detection in clinical diagnostics, forensic science and basic research. Dimensions 77.8 by 130.8 mm. With end tabs removed, length is 118.1 mm with 45° corners.

• ultra-high optical clarity
• fit within plate rim to prevent evaporation due to sealing film lifting
• heat resistant, recommended for temperatures from
  -40 °C to +120 °C
• certified DNase-, RNase-, and nucleic-acid-free

ThermalSeal RTSTM sealing films are based on 50 µm polyolefin films with 50 µm inert encapsulated silicone adhesive. They are optically clear, with low autofluorescence, and are especially suited for real-time qPCR, storage, and protein crystallization applications. The encapsulated silicone adhesive is non-tacky until pressed against the sealing surface, at which time adhesive is released only in sealing areas to form the strongest available heat-resistant seal around each well on the plate. Adhesive on non-sealing areas of the film, such as directly over sample wells, remains encapsulated and inert. Excel ThermalSeal RTS films are sized to fit within the edges of raised-rim 96-well plates. Their consistent high optical clarity makes possible reproducible, reliable, and consistent DNA amplification measurements and crystal detection. Two end tabs assist in positioning the film on the plate, and the non-tacky adhesive surface simplifies handling. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with films that overlap the plate rim. Dimensions 78.6 by 139.7 mm. With end tabs removed, length is 113.0 mm.

• high optical clarity
• minimal to no autofluorescence
• chemically inert; no extractables except at extreme pH
• DMSO resistant for HTS
• heat resistant, recommended for temperatures from
  -70 °C to +100 °C
• certified DNase-, RNase-, and nucleic-acid-free
• fit within raised plate rim to prevent loss of seal due to film lifting
• silicone adhesive forms the strongest available seal for evaporation prevention
• non-tacky adhesive layer simplifies handling of film prior to sealing