| Synonyms |
Acrp30, AdipoQ, GBP-28, APM-1, ACDC. |
| Introduction |
Adiponectin is an important negative regulator in hematopoiesis and immune systems. It may be involved in ending inflammatory responses through its inhibitory functions. Inhibits endothelial nf-kappa-b signaling through a camp-dependent pathway. Inhibits tnf-alpha- induced expression of endothelial adhesion molecules. Involved in the control of fat metabolism and insulin sensitivity. |
| Description |
The Acrp30 Mouse Recombinant is fused with FLAG tag. |
| Source |
HEK293 (Human embryonic kidney cell line). |
| Purity |
Purity of adiponectin mouse recombinant is >98% (HPLC and SDS PAGE analyzed). |
| Biological Activity |
Full-length mouse adiponectin activates AMP-activated protein kinase in hepatocyte and activates AMPK in HepG2 human hepatocytes at a concentration of 1μg/ml.
Mouse adiponectin mammalian cell derived inhibits glucose production as shown by in-vitro gluconeogenesis assay in primary rat hepatocytes. |
| Formulation |
Sterile filtered and lyophilized from 0.5 mg/ml in PBS buffer. |
| Solubility |
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. |
| Stability |
Store lyophilized APM-1 at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
The lyophilized protein remains stable until the expiry date when stored at -20°C. |
| Applications |
ELISA
Western blotting
Cell culture and/or animal studies. |
| Amino Acid Sequence |
EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK. |
| Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |