| Synonyms |
CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Complement-associated protein SP-40, Complement cytolysis inhibitor, NA1/NA2, Apolipoprotein J, Apo-J, Testosterone-repressed prostate message 2, TRPM-2, Sulfated glycoprotein 2, Clu, Apoj, Msgp-2, Sugp-2, AI893575, D14Ucla3. |
| Introduction |
Clusterin mRNA and Clusterin protein are shown to increase with androgen treatment. Binding of clusterin to the LDL-Receptor plays a role in the pathogenesis of membranous glomerulonephritis. Clusterin is down regulated in CaP in association with matched benign controls. Clusterin is involved in cellular senescence and tumorigenesis. Clusterin is involved in photo-oxidative cell death pathway. Clusterin is a functional tumor marker for the diagnosis of pediatric large cell lymphoma. Clusterin is activated in low pH. Clusterin is involved in the inhibition of NF-kappaB signaling through stabilization of IkappaBs thus results in suppression of tumor cell motility. N-terminal deletion of clusterin is vital for its alterations of biogenesis in esophageal squamous cell carcinoma. |
| Description |
Recombinant Mouse Clusterin produced in HEK293 cells is a single, glycosylated, polypeptide chain containing 438 amino acids and having a molecular mass of 50.55kDa. The Mouse Clusterin is fused to 11 amino acid flag tag at c-terminus and purified by proprietary chromatographic techniques. |
| Source |
Human Embryonic Kidney 293 Cells. |
| Physical Appearance |
White lyophilized (freeze-dried) powder. |
| Formulation |
Lyophilized from 0.5 mg/ml containing 20mM Tris pH 7.5 and 20mM NaCl. |
| Solubility |
Add of deionized H2O to a conc. of 0.5mg/ml and let the lyophilized pellet dissolve completely. |
| Stability |
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at -20°C. |
| Purity |
Greater than 95% as determined by SDS PAGE. |
| Applications |
Western blotting. |
| Amino Acid Sequence |
EQEVSDNELQ ELSTQGSRYI NKEIQNAVQG VKHIKTLIEK TNAERKSLLN SLEEAKKKKE DALEDTRDSE MKLKAFPEVC NETMMALWEE CKPCLKHTCM KFYARVCRSG SGLVGQQLEE FLNQSSPFYF WMNGDRIDSL LESDRQQSQV LDAMQDSFAR ASGIIDTLFQ DRFFARELHD PHYFSPIGFP HKRPHFLYPK SRLVRSLMSP SHYGPPSFHN MFQPFFEMIH QAQQAMDVQL HSPAFQFPDV DFLREGEDDR TVCKEIRRNS TGCLKMKGQC EKCQEILSVD CSTNNPAQAN LRQELNDSLQ VAERLTEQYK ELLQSFQSKM LNTSSLLEQL NDQFNWVSQL ANLTQGEDKY YLRVSTVTTH SSDSEVPSRV TEVVVKLFDS DPITVVLPEE VSKDNPKFMD TVAEKALQEY RRKSRAEAAA DYKDDDDK. |
| Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |