上海经科化学科技有限公司 代理美国Solulink公司抗体标
签、抗体-寡肽标签、生物素标签、磁珠及凝胶
欢迎咨询！www.jkchem.cn
产品名称：美国solulink公司ChromaLink? Biotin Labeling Kit生物素标记试剂盒
Convenient kit boosts biotinylation reproducibility
The ChromaLink™ Biotin Labeling Kit provides everything you need for convenient, measurable, antibody or protein biotinylation. The kit contains ChromaLink™ Biotin, which incorporates a UV-traceable chromophore, based on Solulink's proprietary linking technology, to enable reproducibility in your biotinylation process. Now you can measure the degree of biotinylation in minutes, not hours (HABA), without the standard curves required for HABA/avidin and fluoro-reporter assays. With a simple and direct UV scan (A280 and A354), you can quantify biotin incorporation and ensure reproducible production of consistent batches. A large scale kit is also available (labels 2 mg of antibody).
Workflow Diagram
click to expand
Download White Paper: How to Biotinylate with Reproducible Results
The ChromaLink™ Biotin Labeling Kit includes everything you need to biotinylate your antibody: buffers, reagents, spin columns, and an easy-to-follow protocol. With the ChromaLink™ Biotin Labeling Kit, you can biotinylate and purify up to 5 antibodies or proteins ranging in molecular weight from 20–200 kDa from as little as 25 µg to as much as 1 mg in about 2.5 hours. The resulting biotinylated antibody is ready for binding to streptavidin. We recommend our NanoLink™ Streptavidin Magnetic Beads (Cat. No. M-1002-010) with the highest biotin-binding capacity available for assay development.
ChromaLink™ Biotin Labeling Kit Advantages
1. Reproducible Production – UV-traceable chromophore permits nondestructive, rapid A280/A354 quantification of incorporated biotin, enabling consistency and reproducibility
2. Extended PEG3 Spacer – Helps reduce aggregation, minimizes steric hindrance, and enhances solubility
3. Scalable – Labels 25 µg to 1 mg of antibody
4. Convenient – Kit contains buffers, spin columns, and calculator to determine reaction quantities
| |
Solulink ChromaLink™ Biotin Labeling Kit
|
Pierce EZ-Link Sulfo-NHS-Biotinylation Kit
|
Biotinylation Time
|
2.5 hours
|
2.5 hours
|
Quantification of Biotin
|
5 minute UV Scan = Free
|
3 hour HABA Assay = $140
|
| |
Solulink ChromaLink™ Biotin Labeling Kit
|
Pierce EZ-Link Sulfo-NHS-Biotinylation Kit
|
Life Technologies APEX™ Biotin-XX Antibody Labeling Kit
|
Biotin Quantification
|
Yes
|
No
|
No
|
Complete Kit
|
Yes
|
No
|
No
|
Purification
|
Yes
|
No
|
No
|
1.AAdditional Information
1. Protocol
2. Calculator
3. Certificate of Analysis
4. Shipping and Storage Information
5. MSDS
Citations
· Sweetwyne MT, Pallero MA, Lu A, Van Duyn Graham L, Murphy-Ullrich JE. The Calreticulin-Binding Sequence of Thrombospondin 1 Regulates Collagen Expression and Organization During Tissue Remodeling. American Journal of Pathology 2010;177(4):1710-24. Link
· Sharma RK, Schabowsky RH, Srivastava AK, Elpek KG, Madireddi S, Zhao H, Zhong Z, Miller RW, MacLeod KJ, Yolcu ES, Shirwan H. 4-1BB Ligand as an Effective Multifunctional Immunomodulator and Antigen Delivery Vehicle for the Development of Therapeutic Cancer Vaccines. Cancer research 2010;70(10):3945. Link
· Dirksen A, Yegneswaran S, and Dawson PE. Bisaryl Hydrazones as Exchangeable Biocompatible Linkers.Angew. Chem. Int. Ed. 2010,49,2023–2027. Link
· Srinivasan B, Li Y, Jing Y, et al. A detection system based on giant magnetoresistive sensors and high-moment magnetic nanoparticles demonstrates zeptomole sensitivity: potential for personalized medicine.Angew. Chem. Int. Ed. Engl. 2009;48(15):2764-2767.Link
· Ma Z, Li Q, An H, Pankonin MS, Wang J, Loeb JA. Targeting Human Epidermal Growth Factor Receptor Signaling with the Neuregulin's Heparin-binding Domain. Journal of Biological Chemistry 2009;284(46):32108.
相关产品：
货号 B-9007-105K ChromaLink™ Biotin Labeling Kit Kit- labels 25 µg to 1 mg
货号 B-9007-009K ChromaLink™ One-Shot Antibody Biotinylation Kit Kit- Labels 100 µg of Ab
货号 B-1007-110 ChromaLink™ Biotin Labeling Reagent (Water Soluble) 10 mg
货号 B-1007-105 ChromaLink™ Biotin Labeling Reagent (Water Soluble) 5 x 1.0 mg
货号 B-1001-010 ChromaLink™ Biotin (DMF Soluble) 10 mg
货号 B-1001-105 ChromaLink™ Biotin (DMF Soluble) 5 x 1.0 mg
货号 B-1012-010 ChromaLink™ Biotin Maleimide Reagent 10 mg
货号 B-9014-009K ChromaLink™ Digoxigenin One-Shot Antibody Labeling Kit Kit- Labels 100 µg of Ab
货号 B-9014-020K ChromaLink™ Digoxigenin One-Shot Antibody Labeling Kit Large-Scale - Labels 2 mg