上海经科化学科技有限公司 代理美国Solulink公司抗体标签、抗体-寡肽标签、生物素标签、磁珠及凝胶
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产品名称：美国solulink公司HRP-Antibody All-in-One Conjugation Kit辣根过氧化物酶抗体标记试剂盒
No chromatography required
Solulink’s HRP-Antibody All-in-One Conjugation Kit offers an innovative TurboLink™ catalyzed conjugation based on Solulink’s proprietary linking technology that delivers 100% conversion of antibody to conjugate and novel purification that generates a conjugate free of unlabeled antibody or enzyme at a high final yield of >50%, without HPLC. The kit includes everything needed to make direct-labeled two HRP-antibody conjugates from 100 µg of any user-supplied antibody.
Workflow Diagram
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Download White Paper: How to Prepare High-Quality HRP Conjugates
How it works
Solulink’s proprietary linking technology is based on the use of two complementary heterobifunctional linkers:
•S-HyNic (succinimidyl-6-hydrazino-nicotinamide) linker, an NHS-ester, reacts with lysine residues, incorporating HyNic functional groups (hydrazinonicotinamide) onto the antibody.
•Sulfo-S-4FB (sulfo-N-succinimidyl-4-formylbenzamide) linker is conjugated to HRP, providing a pre-activated, high-activity HRP called 4FB-HRP.
•HyNic-modified antibody is incubated with pre-activated 4FB-HRP in the presence of the TurboLink™ catalyst, aniline, leading to rapid and efficient conversion of the antibody to conjugate through formation of stable bis-arylhydrazone bonds.
Catalyzed conjugation. Novel purification.
The efficiency of catalyzed conjugation greatly simplifies conjugate purification. Solulink’s proprietary linking technology is catalyzed by the TurboLink™ catalyst, aniline, under mild reaction conditions, increasing the rate and efficiency, leading to quantitative conversion of free antibody to conjugate. The complete absence of free antibody at the end of the catalyzed reaction leaves only two components; excess HRP and conjugate. After conjugation, a novel Q spin filter is used that quantitatively removes excess HRP to provide high-purity, ready-to-use conjugate. Purified conjugate is then eluted from the filter membrane, free of residual antibody and HRP in high yield (50–70 μg).
HRP-Antibody All-in-One Conjugation Kit Advantages
•Catalyzed Conjugation – Fast catalyzed method generates conjugates in just over 5 hr
•Quantifiable – Conjugate bond is UV-traceable for simple and direct quantification
•High Purity – Novel spin-column purification columns deliver 100% purified conjugates
•Gentle – Amine-reactive chemistry leaves antibodies intact - no reducing reagents
•Easy-to-Use – Protocols and calculators provided to determine MSR
•Versatile – Compatible with western, ELISA, or IHC applications
•Efficient – 100% conversion with 50–70% yields.
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货号 F-9001-009K Fluorescein One-Shot Antibody Labeling Kit Kit - Labels 2 x 100 ug of Ab
货号 B-9007-105K ChromaLink™ Biotin Labeling Kit Kit- labels 25 µg to 1 mg
货号 B-9007-009K ChromaLink™ One-Shot Antibody Biotinylation Kit Kit- Labels 100 µg of Ab
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货号 A-9402-001 RapidDirect Primary Antibody polyHRP Labeling Kit Conjugates 1x100 µg Ab
货号 S-4008-001 4FB Activated R PE 1 mg
货号 S-4008-003 4FB Activated R PE 3 mg
货号 S-4009-001 4FB Activated APC 1 mg
货号 S-4009-003 4FB Activated APC 3 mg