TurboLigationKit（Addingl…

上海经科化学科技有限公司 代理美国Accelagen公司产品

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产品名称：TurboLigation Kit（Adding linkers or adapters to DNA ；Blunt or cohesive end ligation of duplex DNA）

Description:
TurboLigation Kit enables quick ligation of both blunt-end or cohesive-end DNA fragments in just 5 minutes at room temperature. The kit combines TurboLigase^TM, an ultra-pure recombinant T4 DNA Ligase, and 5X TurboLigation Buffer, an optimized quick ligation buffer. A 5 minute TurboLigation reaction offers the same efficiency as a traditional 16^oC overnight ligation reaction. The reaction product is ready for bacterial transformation without any further treatment.

Applications:

• Adding linkers or adapters to DNA
• Blunt or cohesive end ligation of duplex DNA
• DNA cloning

Components:
TurboLigase^TM: 5 unit/µl in Storage Buffer of 10 mM Tris-HCl, pH 7.5, 50 mM KCl, 0.1 mM EDTA, 1 mM dithiothreitol, and 50% glycerol
5X TurboLigation Buffer: 250 mM Tris-HCl, pH 7.5, 50 mM MgCl2, 5 mM dithiothreitol, 5 mM ATP, 25% polyethylene glycol 8000

Stability and Storage:
TurboLigase and 5X TurboLigation Buffer are stable at -20^oC for 12 months from date of receipt.
Store TurboLigase and 5X TurboLigation Buffer at -20^oC.
Shipped in gel packs.

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