德国ZytoVision是世界知名的FISH、CISH检测探针生产厂商,以其质量超凡的稳定性,深受中国高端客户使用者的青睐;香港华创贸易国际有限公司作为德国ZytoVision公司在中国大陆和中国香港的2013-2014年度代理商,热诚为广大客户提供优质的服务。
香港华创贸易国际有限公司及其北京办事处供应EBV病毒 显色原位杂交检测探针及其辅助液试剂盒,质量稳定,操作方便,信号清晰。
1、生物素标记:
1.1、采购指南:
1.1.1、EBV CISH检测探针:
货号:T-1014-400
名称:ZytoFast EBV Probe
标记:Biotin-labeled
规格:400μl
1.1.2、CISH 辅助液试剂盒:
货号:T-1070-40
名称:ZytoFast CISH Implementation Kit AP-NBT/BCIP
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 2x 50 ml;
AP-Streptavidin, 4 ml;
NBT/BCIP Solution, 4 ml
货号:T-1071-40
名称:ZytoFast CISH Implementation Kit HRP-AEC
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 2x 50 ml;
HRP-Streptavidin, 4 ml;
AEC Solution, 4 ml
1.2、文献:
1.2.1、Greifenegger N, et al. (1998) J Virol 72: 9323-8.
1.2.2、Khan G, et al. (1992) J Clin Pathol 45: 616-20.
1.2.3、Khanna R, et al. (1995) Microbiol Rev 59: 387-405.
1.2.4、Murphy JK, et al. (1990) J Clin Pathol 43: 220-3.
1.2.5、Rosa MD, et al. (1981) Mol Cell Biol 1: 785-96.
1.2.6、Thorley-Lawson DA (2001) Nat Rev Immunol 1: 75-82.
Wilkinson DG: In Situ Hybridization, A Practical Approach, Oxford University Press (1992)
ISBN 0 19 963327 4.
2、地高辛标记:
2.1、采购指南:
2.1.1、EBV CISH检测探针:
货号:T-1114-400
名称:ZytoFast EBV Probe
标记:Digoxigenin-labeled
规格:400μl
2.1.2、CISH 辅助液试剂盒:
货号:T-1061-40
名称:ZytoFast PLUS CISH Implementation Kit AP-NBT/BCIP
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Heat Pretreatment Solution EDTA, 500 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 4x 50 ml;
Rabbit-anti-DIG, 4 ml;
Anti-Rabbit-AP-Polymer, 4 ml;
NBT/BCIP Solution, 4ml;
Nuclear Red Solution, 20 ml;
Mounting Solution (alcoholic), 4 ml
货号:T-1062-40
名称:ZytoFast PLUS CISH Implementation Kit HRP-AEC
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Heat Pretreatment Solution EDTA, 500 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 4x 50 ml;
Mouse-anti-DIG, 4 ml;
Anti-Mouse-HRP-Polymer, 4 ml;
AEC Solution, 4 ml;
Nuclear Blue Solution, 20 ml;
Mounting Solution (aqueous), 4 ml
货号:T-1063-40
名称:ZytoFast PLUS CISH Implementation Kit HRP-DAB
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Heat Pretreatment Solution EDTA, 500 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 4x 50 ml;
Mouse-anti-DIG, 4 ml;
Anti-Mouse-HRP-Polymer, 4 ml;
DAB Solution A, 0.3 ml;
DAB Solution B, 10 ml;
Nuclear Blue Solution, 20 ml;
Mounting Solution (alcoholic), 4 ml
货号:T-1073-40
名称:ZytoFast PLUS CISH Implementation Kit HRP-HRP-Green
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Heat Pretreatment Solution EDTA, 500 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 4x 50 ml;
Mouse-anti-DIG, 4 ml;
Anti-Mouse-HRP-Polymer, 4 ml;
HRP-Green-Solution A, 0.8 ml;
HRP-Green-Solution B, 15 ml;
Nuclear Red Solution, 20 ml;
Mounting Solution (alcoholic), 4 ml
货号:T-1151-40
名称:ZytoFast PLUS CISH Implementation Kit AP-Permanent Red
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Heat Pretreatment Solution EDTA, 500 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 4x50 ml;
Rabbit-anti-DIG, 4 ml;
Anti-Rabbit-AP-Polymer, 4 ml;
Permanent Red Solution A, 0.1 ml;
Permanent Red Solution B, 7 ml;
Mayer’s Hematoxylin Solution, 20 ml;
Mounting Solution (alcoholic), 4 ml
2.2、文献:
2.2.1、Greifenegger N, et al. (1998) J Virol 72: 9323-8.
2.2.2、Khan G, et al. (1992) J Clin Pathol 45: 616-20.
2.2.3、Khanna R, et al. (1995) Microbiol Rev 59: 387-405.
2.2.4、Murphy JK, et al. (1990) J Clin Pathol 43: 220-3.
2.2.5、Rosa MD, et al. (1981) Mol Cell Biol 1: 785-96.
2.2.6、Thorley-Lawson DA (2001) Nat Rev Immunol 1: 75-82.
Wilkinson DG: In Situ Hybridization, A Practical Approach, Oxford University Press (1992)
ISBN 0 19 963327 4.
3、相关链接
3.1、香港华创贸易国际有限公司续签为德国ZytoVision公司2013-2014年中国代理商 3.2、德国ZytoVision检测探针现货状态实时显示
3.3、第五届乳腺疾病病理研修班
3.4、ZytoFast CISH 辅助液
3.5、ZytoFast全系列CISH检测探针目录(截止到2013年5月)
3.6、成功案例
3.7、FISH(荧光原位杂交实验)、CISH(显色原位杂交实验)解决方案
4、EBV
EB病毒(Epstein-Barr virus,EBV)是Epstein和Barr于1964年首次成功地将Burkitt非洲儿童淋巴瘤细胞通过体外悬浮培养而建株,并在建株细胞涂片中用电镜观察到疱疹病毒颗粒,故名。
EB病毒在人群中广泛感染,根据血清学调查,我国3~5岁儿童EB病毒VCA-lgG抗体阳性率达90%以上,幼儿感染后多数无明显症状,或引起轻症咽炎和上呼吸道感染。青年期发生原发感染,约有50%出现传染性单核细胞增多症。主要通过唾液传播,也可经输血传染。EB病毒在口咽部上皮细胞内增殖,然后感染B淋巴细胞,这些细胞大量进入血液循环而造成全身性感染。并可长期潜伏在人体淋巴组织中,当机体免疫功能低下时,潜伏的EB病毒活化形成得复发感染。