德国ZytoVision是世界知名的FISH、CISH检测探针生产厂商,以其质量超凡的稳定性,深受中国高端客户使用者的青睐;香港华创贸易国际有限公司作为德国ZytoVision公司在中国大陆和中国香港的2013-2014年度代理商,热诚为广大客户提供优质的服务。
香港华创贸易国际有限公司及其北京办事处供应CMV病毒 显色原位杂交检测探针及其辅助液试剂盒,质量稳定,操作方便,信号清晰。
1、生物素标记:
1.1、采购指南:
1.1.1、CMV CISH检测探针:
货号:T-1013-400
名称:ZytoFast CMV Probe
标记:Biotin-labeled
规格:400μl
1.1.2、CISH 辅助液试剂盒:
货号:T-1070-40
名称:ZytoFast CISH Implementation Kit AP-NBT/BCIP
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 2x 50 ml;
AP-Streptavidin, 4 ml;
NBT/BCIP Solution, 4 ml
货号:T-1071-40
名称:ZytoFast CISH Implementation Kit HRP-AEC
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 2x 50 ml;
HRP-Streptavidin, 4 ml;
AEC Solution, 4 ml
1.2、文献:
1.2.1、Cobbs CS (2002) Cancer Res 62: 3347-50.
1.2.2、Greenaway PJ, Wilkinson GWG (1987) Virus Res 7: 17-31.
1.2.3、Spector SA (1990) Semin Hematol 27 (2 Suppl 1): 11-6; 28-9.
1.2.4、Wu TC, et al. (1992) Am J Pathol 141: 1247-54.
Wilkinson DG: In Situ Hybridization, A Practical Approach, Oxford University Press (1992)
ISBN 0 19 963327 4.
2、地高辛标记:
2.1、采购指南:
2.1.1、CMV CISH检测探针:
货号:T-1113-400
名称:ZytoFast CMV Probe
标记:Digoxigenin-labeled
规格:400μl
2.1.2、CISH 辅助液试剂盒:
货号:T-1061-40
名称:ZytoFast PLUS CISH Implementation Kit AP-NBT/BCIP
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Heat Pretreatment Solution EDTA, 500 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 4x 50 ml;
Rabbit-anti-DIG, 4 ml;
Anti-Rabbit-AP-Polymer, 4 ml;
NBT/BCIP Solution, 4ml;
Nuclear Red Solution, 20 ml;
Mounting Solution (alcoholic), 4 ml
货号:T-1062-40
名称:ZytoFast PLUS CISH Implementation Kit HRP-AEC
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Heat Pretreatment Solution EDTA, 500 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 4x 50 ml;
Mouse-anti-DIG, 4 ml;
Anti-Mouse-HRP-Polymer, 4 ml;
AEC Solution, 4 ml;
Nuclear Blue Solution, 20 ml;
Mounting Solution (aqueous), 4 ml
货号:T-1063-40
名称:ZytoFast PLUS CISH Implementation Kit HRP-DAB
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Heat Pretreatment Solution EDTA, 500 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 4x 50 ml;
Mouse-anti-DIG, 4 ml;
Anti-Mouse-HRP-Polymer, 4 ml;
DAB Solution A, 0.3 ml;
DAB Solution B, 10 ml;
Nuclear Blue Solution, 20 ml;
Mounting Solution (alcoholic), 4 ml
货号:T-1073-40
名称:ZytoFast PLUS CISH Implementation Kit HRP-HRP-Green
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Heat Pretreatment Solution EDTA, 500 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 4x 50 ml;
Mouse-anti-DIG, 4 ml;
Anti-Mouse-HRP-Polymer, 4 ml;
HRP-Green-Solution A, 0.8 ml;
HRP-Green-Solution B, 15 ml;
Nuclear Red Solution, 20 ml;
Mounting Solution (alcoholic), 4 ml
货号:T-1151-40
名称:ZytoFast PLUS CISH Implementation Kit AP-Permanent Red
规格:40人份
组成:DNA (+)-Control Probe, 0.1 ml;
DNA (-)-Control Probe, 0.1 ml;
RNA (+)-Control Probe, 0.1 ml;
RNA (-)-Control Probe, 0.1 ml;
Heat Pretreatment Solution EDTA, 500 ml;
Pepsin Solution, 4 ml;
20x Wash Buffer TBS, 4x50 ml;
Rabbit-anti-DIG, 4 ml;
Anti-Rabbit-AP-Polymer, 4 ml;
Permanent Red Solution A, 0.1 ml;
Permanent Red Solution B, 7 ml;
Mayer’s Hematoxylin Solution, 20 ml;
Mounting Solution (alcoholic), 4 ml
2.2、文献:
2.2.1、Cobbs CS (2002) Cancer Res 62: 3347-50.
2.2.2、Greenaway PJ, Wilkinson GWG (1987) Virus Res 7: 17-31.
2.2.3、Spector SA (1990) Semin Hematol 27 (2 Suppl 1): 11-6; 28-9.
2.2.4、Wu TC, et al. (1992) Am J Pathol 141: 1247-54.
Wilkinson DG: In Situ Hybridization, A Practical Approach, Oxford University Press (1992)
ISBN 0 19 963327 4.
3、相关链接
3.1、香港华创贸易国际有限公司续签为德国ZytoVision公司2013-2014年中国代理商 3.2、德国ZytoVision检测探针现货状态实时显示
3.3、第五届乳腺疾病病理研修班
3.4、ZytoFast CISH 辅助液
3.5、ZytoFast全系列CISH检测探针目录(截止到2013年5月)
3.6、成功案例
3.7、FISH(荧光原位杂交实验)、CISH(显色原位杂交实验)解决方案
4、CMV
巨细胞病毒(Cytomegaoviyns,CMV)是一种疱疹病毒组DNA病毒。分布广泛,其他动物皆可遭受感染,引起以生殖泌尿系统,中枢神经系统和肝脏疾患为主的各系统感染,从轻微无症状感染直到严重缺陷或死亡。巨细胞病毒(CytomegalovirusCMV)亦称细胞包涵体病毒,由于感染的细胞肿大,并具有巨大的核内包涵体,故名。