Immobiline™ DryStrip Gels

• Immobiline™ DryStrip gels (IPG strips) are used for isoelectric focusing (IEF), run as the first dimension of 2-D electrophoresis or as a separate application.
• Immobiline™ DryStrip gels have been improved on several levels, through new process parameters, to produce first and second dimension gels of overall higher quality.
• Improved Immobiline™ DryStrip gels provide increased stability, reproducibility, and protein spot definition.
• Better spot definition improves spot quantitation in data analysis.
• Wide selection of IPG strips, pH gradients, lengths, and buffers for greater versatility.
• Immobiline™ DryStrip gels with matching IPG Buffer gives a high sample loading capacity and improves resolution

Immobiline™ DryStrip Gels

Technical Information

Immobiline™ DryStrip pH 4-7 run according to standard procedures.
A) Spots are elongated in the second dimension due to skewed bands in the first dimension.
B) Improved Immobiline™ DryStrip gels provide a more stable pH gradient and better spot definition in the second dimension.

Immobiline™ DryStrip gels (IPG strips) are used for isoelectric focusing (IEF), run as the first dimension of 2-D electrophoresis or as a separate application. IPG strips contain a preformed pH gradient immobilized in precast polyacrylamide gels on a plastic backing. A comprehensive range of overlapping IPG strips covering narrow, medium, and wide pH ranges are available in various strip lengths, and the strips are individually packaged and bar coded for convenient handling and sample tracking.

Immobiline™ DryStrip pH 3-11 NL covers most proteins found in prokaryotic and eukaryotic cells, while the narrow-range strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear (NL) to distribute the proteins evenly over the gel length and obtain maximal resolution.

Immobiline™ DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of protein. Samples can also be loaded after rehydration. The first dimension separation is carried out using Ettan™ IPGphor™ 3 IEF system or Multiphor™ II flatbed system. When used for 2-D DIGE applications, the gels give accurate and reliable 2-D results.

SELECTION GUIDE - Immobiline™ DryStrip Gels and IPG Buffers

Guidelines for choosing Immobiline DryStrip gels
 

• Use shorter strips for fast screening or when only the most abundant proteins are of interest.
   
• Use longer strips for maximal resolution and loading capacity.
   
• To overview total protein distribution, use pH 3-11 NL strips.
   
• For increased resolution between pH 5-7, use a nonlinear gradient pH 3-10 strip to distribute the proteins more evenly over the strip.
   
• Combine pH 3-7 NL and pH 6-11, pH 3-7 NL and pH 6-9, or select from 3-5.6 NL, 5.3-6.5, 6.2-7.5, and 7-11 NL when more detail is required.
   
• Use narrow pH range strips (1 pH unit) to closely study proteins in the regions of interest.

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