PKA-322,PRKCEHuman,重组人…

PKA-322

 现货

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.

Manual

SynonymsTop Protein kinase C epsilon type, EC 2.7.11.13, nPKC-epsilon, PRKCE, PKCE, MGC125656, MGC125657. Source Sf9 insect cells. Physical AppearanceTop Sterile Filtered clear solution. Unit DefinitionTop 3250 units/mg. One unit of PRKCE activity transfers 1 nmole of phosphate to a PRKCE substrate peptide per minute at 30°C. Recombinant human active PRKCE also phosphorylates full length myelin basic protein, MARKS, GAP-34, and raf-1 kinase. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. Storage ConditionsTop Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. PurityTop Greater than 90% as determined by SDS-PAGE. UsageTop Prospec' s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.