美国创新技术DO400光纤测氧仪
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美国创新技术DO400光纤测氧仪--不须更换电解膜,填充电解液和进行费时的极化,不耗氧,无流动干扰
美国创新技术DO400光纤测氧仪
AH Medical DO400 Fiber Optic Oxygen Sensor System 2009 08.pdf
DO400光纤测氧仪是基于荧光质的荧光动态淬灭反应原理,将荧光质与透氧高分子膜基质一起固定于光纤探头表面.通过应用本公司专利申请的脉冲荧光测定技术,所有出现在长期连续监测氧的问题包括荧光质活性减退问题都得到了有效控制.基于此,DO400光导纤维测氧仪可以保证最优良的稳定性和最低的检测飘移.
DO400光纤测氧仪是为长期在线检测,监控,和存档记录溶解氧气和空气中含氧量数据而设计的. 仪器光纤探头具有不耗氧和不受溶液流动状态干扰影响的特点,使该仪器还能应用于高粘度溶液和样品中氧的连续接触监测. 该仪器还具有很高的灵敏度和快速的响应时间. 除此而外,该仪器还具有抗电子干扰,抗溶液pH,盐度,离子强度改变和生物污垢等干扰的优点. 校正极为方便,只须简单的一点法校正(100%空气饱和的水中或空气中).
该仪器可应用于生物化学技术(细胞培养, 发酵,和生物样品), 医药研究, 高校及科研实验室, 生物耗氧量 (BOD), 食品和饮料生产业(食品包装和软饮料装瓶),空气中氧等.
DO400光纤测氧仪是由计算机软件控制并在计算机Window支持的环境下操作. 软件设计非常方便用户. 使用者可以很容易地校正仪器,采集和展示数据,改变参数和处理数据.
仪器采用六种不同的单位显示测氧结果,它们包括:氧气偏压(mmHg,毫米汞柱),氧气偏压(mbar,毫巴), 空气饱和度(%Air),氧气饱和度(%O2),毫克/升(mg/L,即ppm),和mM. 采用多个虚拟长图记录仪连续显示测定数据走向.对压力,温度,相对湿度及盐度的补偿是该仪器的标准功能.该测氧仪非常方便地通过USB(通用窜行总线)与计算机联接,不须在计算机上安装任何功能卡, 是真正的接插即操作系统.
光纤测氧探头是一种固体探头,是由316不锈钢管和石英光纤芯组成. 探头不须填充电解液,不须更换电极膜,以及不须费时进行极化.测氧探头通过一条光缆与测氧仪连接,并采用标准的SMA905接口联接. 仪器不须再另外联接独立的光源,不须使用Y型光缆.
测氧仪同时还具有测温功能. 仪器配接了一个RTD(Resistance-Temperature Detector, 电阻温度探测器) 测温探头,以便在测氧同时精确记录温度和进行温度补偿. 测氧探头安装在一个316不锈钢管内,通过一个标准的1/4英寸耳机插座与测氧仪联接. 测氧探头和测温探头均采用316不锈钢材料制成,使得测氧仪适合生物环境检测. 因此,该仪器可安全使用于医疗,细胞培养及发酵中.
仪器优点及特长
基于荧光动态淬灭原理
固态测氧探头
脉冲荧光测定技术
· 低维护,无须更换电解膜,填充电解液和进行费时的极化.
· 不耗氧,无流动干扰,使其能检测高粘度样品
· 高灵敏度和快速的响应时间.
· 抗干扰好,对电场及磁场,不同化学品及气体环境,和pH值,盐度,离子强度的改变以及污垢均有免疫性.
· 优秀的长期稳定性.
· 无杂散光干扰.
· 不须另接独立的光源,不须使用Y型光缆.
· 检测探头不须保持润湿,使其既可检测溶解氧,又可检测气态氧.
· 可用EtO(氧化乙烯),双氧水,高緑酸钠,和Gamma射线无菌消毒.
· 使用寿命长. 能适用于生物环境,探头部分均采用316不锈钢材料制
成.
高性能,多功能
精确测温功能
· 简易的两点校正法: 100%和0%空气饱和的水,或在空气/氮气中.
· 六种不同单位同时显示测氧结果: pO2(mmHg,毫米汞柱),pO2 (mbar,
毫巴),%air sat(空气饱和度), %O2 sat(氧气饱和度),mg/L(即
ppm),和mM.
· 对压力,温度,盐度,相对湿度影响有效地补偿.
· RTD精确地测定温度,并能自动地对温度进行补偿
· 具备气体压力单位换算功能和溶液电导计算盐度功能.
· 对氧和温度测定值超过最高及最低点有警报功
通过外接主控PC计算机
的视窗控制
· 易操作,操作者通过计算机屏幕上的虚拟仪器表盘进行操作.
· 直接存档记录数据,所有采集数据都以ASCll码记录并附有采集时间和日期以便日后回访.
· 所有检测数据及参数都以数字及虚拟长图记录仪显示在计算机屏幕
上.
· 校正仪器及改变参数极其方便.
仪器通过USB接口与主控计算机连
接
· 支持热接插,真正的接插即操作!
· 安装极其容易简单.
· 无须在主控计算机上外接任何功能卡.
· 超强快速的仪器控制,数据采集及数据处理功能.
部分用户名单:
哈佛大学, 哥仑比亚大学,匹兹堡大学医疗中心,
伊利诺斯大学, 夏威夷大学,
美国太平洋西北国家实验室,
爱尔康实验室, 奥克拉哈马大学,
玻德因学院, 纽约斯东尼伯鲁克大学,
塔托大学(立陶宛), 等等。
Specification of Model DO400 Fiber Optic Oxygen Sensor
DO400 Oxygen Sensor (DO400)
Dynamic Range
0-480 mmHg, 0-25 ppm (mg/L), 0%-300% air saturation
Accuracy
1% of reading or 0.02 ppm, whichever is greater
Response Time (90%)
< 15 s
Resolution
0.01 ppm
Stability (per 24 hr period)
0.01 ppm
Repeatability
0.01 ppm
Detection Limit
0.02 ppm
Operating Temperature Range
0 - 70 C
Calibration Method
One-point (100% medium) and optional two-point (0% and 100%
media)
Compensation Method
Temperature - automatic measured from temperature probe
Pressure
Manually input
Relative humidity
Manually input
Salinity
Manually input salinity or conductivity
Ambient Condition Requirements
Temperature: -20 to +70 C
Humidity
0 to 100 percent
Pressure
100 psig
Operation
Controlled via a software under Window enviroment
Display
Virtual trend charts and digital indicators on Window
Data Log
ASCII file, with date, time, and hour traceable
Data Refresh Frequency
Minimum 1 second and maximum 600 seconds
Communication
USB connector, Plug and Play
Power Requirements
9V DC/100 mA, regulated
Enclosure
Aluminum (22 x 10 x 5 cm3 = L x W x H); Weight 0.6 Kg
DO Probe (DOProbe-100, -200, -18G4, -2000, -4000)
DO Sensing Material
Fluorophores immobilized in a polymeric thin-film matrix
coated on the probe tip
DO Probe Principle
Fiber optic sensor based on dynamic fluorescence quenching
DO Probe Material
316 stainless steel and silica optical fiber core (1000 um OD)
DO Probe Size
DOProbe-100: 18 cm length and 1.6 mm OD
DOProbe-200: 6.4 cm length and 3.18 mm OD
DOProbe-18G4: 4 inch (10 cm) length and 18 gauge (1.26 mm) OD
DOProbe-2000: 14 cm length and 3.18 mm OD
DOProbe-4000: 18 cm length and 6.35 mm OD
Connector
Stainless steel SMA 905
Optical Fiber Cable
2 meters length, 1000 um OD silica optical fiber core with multiple
buffers and protected by a PVC-Kevlar composite jacketing
Sterilization
EtO, H2O2, sodium hypochlorite, and Gamma radiation
Electrode
No electrode
Electr, olyte
No electrolyte
Membrane Cartridge
No membrane
Minimum Sample Flow Rate
No flow requirements
Drift
Less than 1%
Temperature Probe (TProbe-403 and embedded in DOProbe)
Temperature sensor principle
Precision NTC thermistor
Probe Material
316 stainless steel ferrule
Probe size
13.5 cm length and 3.8 mm OD
Connector
1/4” phone plug
Cable length
10 feet
Measuring Range
-40 to 150 0C
Accuracy
+0.1 0C
Resolution
0.01 0C
Stability
Less than 0.2 0C / 10 month at 100 0C
Interchangeability
+0.2 0C
Time Constant
3.4 seconds
Traceability
Probe traceable to the NIST
Isolation
Probes are electrically-isolated
Biocompatibility
Biocompatible; probes are the world standard for medical use in
humans
What is the difference between the DO400 Fiber Optic Oxygen Sensor and other optical oxygen
sensors in the market today?
DO400 is developed based on our advances in pulsed fluorescence technologies, therefore it
guarantees excellent long-term sensor stability without drifting. It is designed by experts who have
extensive experiences in DO measurement and instrumentation, so its function and performance are
outstanding and will satisfy the needs of different users. DO400 uses USB-based data acquisition, so
it is very easy to install. In addition, the DO400 software is very user-friendly and extremely easy to use.
Overall, DO400 has better quality and performance but much lower price!
What can be measured with a DO400 Fiber Optic Oxygen Sensor?
Oxygen partial pressure (pO2, mmHg) and temperature of an aqueous or gaseous sample can be
directly measured; oxygen concentration (mg/L, ppm, and mM) and percent saturation (%AIR and
%O2) are calculated based on oxygen solubility at known pO2, temperature, salinity, and pressure.
Oxygen partial pressure is actually the Oxygen Activity (or called Fugacity). No mater using an optical
sensor or an electrochemical sensor, the directly measurable parameter is always the oxygen activity,
i.e., partial pressure. At the same oxygen partial pressure, its oxygen concentration (mg/L or mM) can
vary depending on the temperature and salinity, since those two parameters will affect the solubility of
oxygen in water.
Do I need buy any other cards, light source, bifurcated optical cable, temperature control box, etc,
to make the sensor work?
NO! Those are required by products only from other competitors such as Ocean Optics, Avantes, etc.
DO400 makes life much easier: DO400 Integrates light source and temperature control into one
single box, therefore No need of Y-type bifurcated optical cable and No need of any separate cards,
boxes, light source, etc.
Is setting up the DO400 system simple?
Yes, as simple as you can think. Just plug the USB cable, insert the software CD, and the system will
work in less than 5 minutes.
How to calibrate the sensor? Is it easy?
Calibrating DO400 oxygen sensor is as easy as a, b, c. The oxygen probe is manufacturer
pre-calibrated and a calibration code is , provided; therefore the user just need to perform a simple
one-point calibration in a 100% medium (refer to air percent). An optional two-point calibration in
0%AIR and 100%AIR media also provided. Click, ing the [Calibration] button in the measurement
panel will pop up a calibration panel and guide you through the process.
What is the optical oxygen probe lifetime?
In normal operation condition the probe has at least one year lifetime without the need for any
replacement. However, if under long-term continuous operation at extreme condition such as over 80
Celsus, the probe life may shorten.
Do you have any service for probe replacement?
When comes the need of replacement of the probe, Golden Scientific will do re-coating of the probe at
the charge of $200 service fee plus $5 shipping. It will be very easy, just send us your probe, and it will
have a new coating and return to you in about 10 days.
What about if I want to customer make a probe with specified length of optical cable?
The standard length of optical cable is two meters. We can customer make any length specified by
you at the cost of $50/per additional meter covering the material; no additional service cost.
If you need to customer make any special probe other than our standard size (the OD and length of
the S.S. ferrule or needle), please call Golden Scientific, we can do it for you. Generally we will charge
certain amount of service fee plus the material in addition to the standard price.
What is the principle of oxygen measurement based on fluorescence quenching?
Optical oxygen sensors are based on the property of molecular oxygen quenching of fluorescence.
The luminescent probe molecules are encapsulated in a gas permeable, but ion impermeable,
material and coated on the optical probe tip.
Upon irradiation of the luminescent molecules by an excitation light (blue), a fluorescence (red) emits
from the probe tip.
In the presence of oxygen, the fluorescence is reversibly and quantitatively quenched by oxygen due to
form charge transfer complex. Reversible quenching of the luminescent dyes by oxygen has been
found to obey the Stern-Volmer equation:
I0/I = 1 + Ksv*pO2 (1)
where Io and I are the intensities of the fluorescence in the absence and presence, respectively, of
oxygen at partial pressure pO2, and Ksv is the Stern-Volmer constant. The Ksv depends directly upon
the rate constant for the diffusion of oxygen, the solubility of oxygen, and the natural lifetime of the
electronically excited state of the lumophore in the matrix. Equation 1 applies only to a homogeneous
solution containing the luminescent dye and oxygen.
Above equation indicates that, by measuring the fluorescences in two medium containing zero oxygen
and known oxygen level (pO2), the Ksv can be determined; this is achieved during sensor calibration.
After sensor calibration, with the Ksv and I0 known, the oxygen partial pressure of a sample can be
determined by measuring the fluorescence of the probe immersed in the sample.
In most of the luminescent oxygen sensors developed to-date, where the lumophore is incorporated
into a polymer matrix, it has been observed that, in contrast to homogeneous solution, the
Stern-Volmer plot of I0/I versus pO2 has a downward curvature. Therefore, in the real situation, a
modified Stern-Volmer equation that still maintains a linear relationship between pO2 and f(I0, I) is
used.
What is the advantage of an optical sensor over the traditional electrochemical sensor?
The advantage of an optical oxygen sensor over its counterpart electrochemical one is determined by
their intrinsic principles: the electrochemical sensor measures the current generated by reduction of
oxygen at the working electrode, therefore it consumes oxygen; in contrast, the optical sensor does
not consume oxygen.