The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. This convenient "add-and-read" system generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. The Dual-Glo® Luciferase Assay System provides high Z´-factors for cell-based, high-throughput screening applications. With the Dual-Glo® System, internal controls can be established to minimize sample variability by reducing false positive and false negative readings ca...
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Dual-Glo® Luciferase Assay System(Dual-Glo® 双萤光素酶检测系统) 是一种均质检测系统，能够快速简便地从一个样品中定量两个报告基因的稳定的萤光信号。这种方便的“加样- 检测”系统可直接用于未经过预处理或预裂解的细胞，产生萤火虫和海肾萤光素酶两种发光信号。Dual-Glo® 双萤光素酶检测系统为基于细胞的高通量筛选提供了高Z` 因子。Dual-Glo® 系统可以构建内对照来减少样品间的差异，因为内对照能减少非特异性因素( 如细胞毒性) 造成的假阳性和假阴性结果。在Dual-Glo® 双萤光素酶检测中，主报告基因的活性与特异性刺激互相影响，而共转染的对照报告基因活性提供了一个归一化的内对照。此系统是为批处理96 孔和384 孔板而优化设计，与大多数哺乳动物细胞培养基兼容。
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