热卖产品: SuperHoTTaq DNA polymerase
Currently the SuperHoTTaq DNA polymerase from Bioron is regarded as one of the best
enzymes for specific and sensitive qPCR.
SuperHotTaq (Hot Start) DNA Polymerase is the optimized mixture of Taq Polymerase and Anti-Taq monoclonal antibodies. Antibodies block polymerase activity during set-up of the PCR reactions at ambient temperature (20-22°C). The inhibition of Taq DNA polymerase is completely reversed when the temperature is above 70°C. The PCR products obtained with SuperHotTaq are free from unspecific products and from primer-dimers.
特点:
- reliable and reproducable quantification in qPCR
- perfect for Real Time PCR
- especially for diagnostic purposes
- reaction set-up at room temperature
- activation of enzyme during first heating
- no change or optimization of protocol necessary
- high specifity, reduced primer mismatch or dimers
浓度:
5 units/µl (or high concentrated 17.5 u/µl)
Storage buffer:
10 mM Tris-HCI (pH 7.0)
50 mM KCl
0.1 mM EDTA
50% glycerol
from left to right:
1. 100bp DNA ladder
2. Negative control
3. M. genitalium DNA, no dilution
4. M. genitalium DNA 10-1 dilution
5. M.genitalium DNA 10-2 dilution
6. M. genitalium DNA 10-3 dilution
7. M. genitalium DNA 10-4 dilution
更多信息详见:SuperHoTTaq DNA聚合酶