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抗体信息如下：

Primary Antibodies

ASK1 Antibody

Background

Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5 (1-3). ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-Α-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.

Additional Names

ASK1 (C2), Apoptosis signal-regulating kinase, MAPKKK, MEK kinase 5

	Description Left: Western blot analysis of ASK1 in SW1353 cell lysate with ASK1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml. Below: Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 2 μg/ml.
	Other Product Images

Source

ASK1 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human ASK1.

Purification

Affinity chromatography purified via peptide column

Clonality / Clone

This is a polyclonal antibody.

Host

ASK1 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Human ASK1 Peptide (C2) (Cat. No. 3677P)

Application

ASK1 antibody can be used for the detection of ASK1 by Western blot at 1 ?2 μg/ml.

Tested Application

E, WB, ICC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat. No. 3677P - ASK1 Peptide

Storage

ASK1 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1214 - SW1353 Cell Lysate
2. Cat. No. 1202 - A431 Cell Lysate

Species Reactivity

H, M

Protein GI Number

66391167

Protein Accession Number

BAA12684

Short Description

(C2) Apoptosis signal-regulating kinase

This product belongs to the following categories:

• Antibodies
• Polyclonal Antibodies
• Apoptosis Antibodies

Related Products

• A-431 Cell Slide
  (Catalog No. 17-002).
• A431 Lysate
  (Catalog No. 1202).
• ASK1 Antibody
  (Catalog No. 1151).
• ASK1 Antibody
  (Catalog No. 3679).
• ASK1 Peptide (C2)
  (Catalog No. 3677P).
• Daxx Antibody
  (Catalog No. 1163).
• SW1353 Lysate
  (Catalog No. 1214).

References

1. Ichijo H, Nishida E, Irie K, et al. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 1997; 275:90-4.
2. Wang XS, Diener K, Jannuzzi D, et al. Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase. J. Biol. Chem. 1996; 271:31607-11.

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