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抗体信息如下：
Primary Antibodies
Bid Antibody
Background
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death (reviewed in 2 and 3). Bid, a pro-apoptotic Bcl-2 family member, is cleaved by caspase-8 in response to apoptotic signals (4,5), exposing the Bcl-2 homology 3 (BH3) domain which is normally buried in the full-length protein (6). The cleaved complex is myris-toylated and translocated to the mitochondrial membrane where it may induce mitochondrial Bax and Bak to oligomerize (7,8).
Additional Names
Bid (CT), Bid
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Description
Left: Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunohistochemical staining of mouse lung tissue using Bid antibody at 2 μg/ml. |
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Other Product Images
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Source
Bid antibody was raised against a peptide corresponding to 14 amino acids near the carboxy-terminus of human Bid.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
Bid antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Bid (C-Terminus) Peptide (Cat. No. 3353P)
Application
Bid antibody can be used for detection of Bid by Western blot at 0.5 to 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3353P - Bid Peptide
Storage
Bid antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1402 - Mouse Lung Tissue Lysate
Species Reactivity
H, M
Protein GI Number
54673639
Protein Accession Number
AAH36364
Short Description
(CT) a member of the bcl-2 family
This product belongs to the following categories:
- Antibodies
- Polyclonal Antibodies
- Apoptosis Antibodies
- Cancer Antibodies
Related Products
- Bak Antibody
(Catalog No. 3347).
- Bax Antibody
(Catalog No. 3351).
- Bcl-2 Antibody
(Catalog No. 3335).
- Bid Antibody
(Catalog No. 3355).
- BID Antibody
(Catalog No. 45-021).
- Bid Peptide
(Catalog No. 3353P).
- Lung Lysate
(Catalog No. 1402).
- Multiple Species Tissue Array - Lung Tissue
(Catalog No. 10-100-MSTA).
References
- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
- Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
- Wang K, Yin XM, Chao DT, et al. BID: a novel BH3 domain-only death agonist. Genes Dev. 1996; 10:2859-69.
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