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抗体信息如下：
Primary Antibodies
Bcl-2 Antibody
Background
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis (reviewed in 2 and 3). These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis (4). It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad (5 for review). Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer (4,6).
Additional Names
Bcl-2 (NT), Bcl-2
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Description
Left: Western blot analysis of Bcl-2 in Daudi cell lysates with Bcl-2 antibody at (A) 1, (B) 2, and (C) 4 μg/ml.
Below: Immunohistochemical staining of human kidney using Bcl-2 antibody at 2 μg/ml. |
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Other Product Images
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Source
Bcl-2 antibody was raised against a peptide corresponding to 15 amino acids near the N-terminus of human Bcl-2.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
Bcl-2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Murine Bcl-2 (N-Terminus) Peptide (Cat. No. 3335P)
Application
Bcl-2 antibody can be used for detection of Bcl-2 by Western blot at 1 to 2 μg/ml. Bcl-2 antibody can also detect Bcl-2 by immunohistochemistry at 2 μg/ml. It is human and mouse reactive.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3335P - Bcl-2 Peptide
Storage
Bcl-2antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1224 - Daudi Cell Lysate
- Cat. No. 1305 - Human Kidney Tissue Lysate
Species Reactivity
H, M
Protein GI Number
20072668
Protein Accession Number
AAH27258
Short Description
(NT) a member of the bcl-2 family
This product belongs to the following categories:
- Antibodies
- Polyclonal Antibodies
- Apoptosis Antibodies
- Autophagy Antibodies
- Cancer Antibodies
Related Products
- BAD Antibody
(Catalog No. 3343).
- Bak Antibody
(Catalog No. 3347).
- Bcl-2 Antibody
(Catalog No. 3337).
- Bcl-2 Peptide
(Catalog No. 3335P).
- Daudi Lysate
(Catalog No. 1224).
- Kidney Lysate
(Catalog No. 1305).
- Kidney Tissue Slide (Normal)
(Catalog No. 10-401).
References
- Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
- Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607.
- Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
- Hockenbery D, Nunez G, Milliman C, et al. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 1990; 348:334-6.
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