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抗体信息如下：
Primary Antibodies
Bcl-B Antibody
Background
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-B is a recently discovered anti-apoptotic member of the Bcl-2. Unlike the mouse homolog (also known as Diva/Boo) which is predominantly expressed in ovary and testis, the human Bcl-B protein is widely expressed. Also, the human Bcl-B functions by binding to and suppressing the apoptotic activity of Bax, whereas the mouse homolog binds Bak and also interacts with the apoptosis protein Apaf-1. Despite its predicted molecular weight, Bcl-B is often at higher molecular weights, presumably due to post-translational modifications.
Additional Names
Bcl-B, Boo, Diva, Nrh
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Description
Left: Western blot analysis of Bcl-B in Jurkat lysate with Bcl-B antibody at 1 礸/ml in the (A) absence and (B) presence of blocking peptide.
Below: Immunocytochemistry of Bcl-B in Jurkat cells with Bcl-B antibody at 10 μg/ml. |
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Other Product Images
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Source
Bcl-B antibody was raised against a 16 amino acid peptide from near the amino terminus of human Bcl-B.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Bcl-B antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Bcl-B antibody can be used for the detection of Bcl-B by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 3163P - Bcl-B Peptide
Storage
Bcl-B antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Protein GI Number
9966783
Protein Accession Number
NP_065129
Short Description
an anti-apoptotic member of the Bcl-2 family
This product belongs to the following categories:
- Antibodies
- Polyclonal Antibodies
- Apoptosis Antibodies
Related Products
- Apaf1 Antibody
(Catalog No. 2015).
- Bak Antibody
(Catalog No. 3347).
- Bax Antibody
(Catalog No. 3351).
- Bcl-2 Antibody
(Catalog No. 3335).
- Bcl-B Peptide
(Catalog No. 3163P).
- Jurkat Cell Slide
(Catalog No. 17-005).
- Jurkat Lysate
(Catalog No. 1205).
References
- Cory S, Huang DCS, and Adams JM. The Bcl-2 family: roles in cell survival and oncogenesis. Oncogene 2003; 22:8590-607
- Heiser D, Labi V, Erlacher M, et al. The Bcl-2 protein family and its role in the development of neoplastic disease. Exp. Geron. 2004; 39:1125-35.
- Ke N, Godzik A, and Reed JC. Bcl-B: A novel Bcl-2 family member that differentially binds and regulates Bax and Bak. J. Biol. Chem. 2001; 276:12481-4.
- Song Q, Kuang Y, Dixit VM, et al. Boo, a negative regulator of cell death, interacts with Apaf-1. EMBO J. 1999; 18:167-78
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