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抗体信息如下：
Primary Antibodies
RIP3 Antibody
Background
Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNFR1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3 (1-3). RIP3 contains N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNFR1, mediates TNFR1 induced apoptosis, and attenuates RIP and TNFR1 induced NF-kB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues (2,3).
Additional Names
RIP3
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Description
Left: Western blot analysis of RIP3 in mouse 3T3 whole cell lysate in the absence (A) or presence (B) of blocking peptide with RIP3 antibody at 1 μg/ml.
Below: Immunohistochemistry of RIP3 in rat kidney tissue with RIP3 antibody at 5 μg/ml. |
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Other Product Images
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Source
RIP3 antibody was raised against a peptide corresponding to amino acids 473 to 486 of murine RIP3.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
RIP3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human RIP3 Peptide (Cat. No. 2283P)
Application
RIP3 antibody can be used for detection of RIP3 by Western blot at 0.5 to 1 μg/ml. An approximately 57 kDa band can be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2283P - RIP3 Peptide
Storage
RIP3 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1212 - 3T3 Cell Lysate
Species Reactivity
M, R
Protein GI Number
6063101
Protein Accession Number
AAF03133
Short Description
Mouse Receptor Interacting Protein 3
This product belongs to the following categories:
- Antibodies
- Polyclonal Antibodies
- Apoptosis Antibodies
Related Products
- 3T3 (Balb) Lysate
(Catalog No. 1212).
- 3T3/NIH Cell Slide
(Catalog No. 17-202).
- RIP3 Peptide
(Catalog No. 2283P).
References
- Yu PW, Huang BC, Shen M, Quast J, Chan E, Xu X, Nolan GP, Payan DG, Luo Y. Identification of RIP3, a RIP-like kinase that activates apoptosis and NFκB. Curr Biol. 1999;9(10):539-42.
- Sun X, Lee J, Navas T, Baldwin DT, Stewart TA, Dixit VM. RIP3, a novel apoptosis-inducing kinase. J Biol Chem. 1999;274(24):16871-5.
- Pazdernik NJ, Donner DB, Goebl MG, Harrington MA. Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-κB. Mol Cell Bio. 1999; 19(10):6500-8 (WD0102
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