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抗体信息如下：
Primary Antibodies
Caspase-9 Antibody
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3 (1-3). Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation (3). Caspase-9 is also activated by granzyme B and CPP32 (1,2). Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD (4). Caspase-9 is expressed in a variety of human tissues (1,2).
Additional Names
Caspase-9 (IN2), Mch6/ Apaf-3, Casp-9
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Description
Left: Western blot analysis of caspase-9 in HeLa whole cell lysate with Caspase-9 antibody at 1:1000 dilution
Below: Immunocytochemistry staining of K562 cells using Caspase-9 antibody at 2 μg/ml. |
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Other Product Images
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Source
Caspase-9 antibody was raised against a peptide corresponding to amino acids 299 to 318 of human caspase-9 .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Caspase-9 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Caspase-9 (Intermediate Domain 2) Peptide (Cat. No. 2073P)
Application
Caspase-9 antibody can be used for detection of caspase-9 by Western blot at 1:1000 to 1:2000 dilution. A 46 kDa band should be detected. It has no cross response to other members in caspase family.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2073P - Caspase-9 Peptide
Storage
Caspase-9 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H
Protein GI Number
28558771
Protein Accession Number
P55211
Short Description
(IN2) A new caspase
This product belongs to the following categories:
- Antibodies
- Polyclonal Antibodies
- Apoptosis Antibodies
- Cancer Antibodies
Related Products
- Apaf-1 Monoclonal Antibody
(Catalog No. PM-2015).
- Apaf-1 Monoclonal Antibody
(Catalog No. PM-2016).
- Caspase-9 Peptide
(Catalog No. 2073P).
- HeLa Cell Slide
(Catalog No. 17-001).
- HeLa Lysate
(Catalog No. 1201).
References
- Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He WW, Dixit VM. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J Biol Chem 1996;271:16720-4
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