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抗体信息如下：
Primary Antibodies
Bim Antibody
Background
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines (1,2).
Additional Names
Bim (IN), BOD
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Description
Left: Western blot analysis of Bim in K562 (K) and A549 (A) whole cell lysates with Bim antibody at 1 μg/ml.
Below: Immunohistochemistry of Bim in human skin cancer cells with Bim antibody at 20 μg/ml. |
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Other Product Images
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Source
Bim antibody was raised against a peptide corresponding to amino acids near the center of human Bim. The sequence is identical to that of mouse and differs from that of rat by one amino acid .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Bim antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Bim (Intermediate Domain) Peptide (Cat. No. 2065P)
Application
Bim antibody can be used for detection of Bim by Western blot at 1 μg/ml dilution. A 23 kDa band can be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2065P - Bim Peptide
Storage
Bim antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
18202042
Protein Accession Number
O43521
Short Description
(IN) A new member in Bcl-2 family
This product belongs to the following categories:
- Antibodies
- Polyclonal Antibodies
- Apoptosis Antibodies
Related Products
- BIM Antibody
(Catalog No. 45-330).
- Bim Peptide
(Catalog No. 2065P).
- K-562 Cell Slide
(Catalog No. 17-004).
- K562 Lysate
(Catalog No. 1204).
References
- O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395.
- Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.
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