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乐博现货供应美国ProSci凋亡相关--ATG16(NT)antibody
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  • 参考报价:2950 产地:北京北京 品牌:ProSci 型号:4425 更新时间:2012/7/18

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抗体信息如下:

Primary Antibodies

ATG16 Antibody

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.

 

Additional Names

ATG16 (NT), Autophagy protein 16, Autophagy related protein 16, ATG16L, APG16

ATG16 Antibody

Description

Left: Western blot analysis of ATG16 in HeLa cell lysate with ATG16 antibody at (A) 1 and (B) 2 μg/ml.

Below: Immunocytochemistry of ATG16 in HeLa cells with ATG16 antibody at 2 μg/ml.
 

Other Product Images

Source

ATG16 antibody was raised against a 18 amino acid peptide from near the amino terminus of human ATG16.

Purification

Affinity chromatography purified via peptide column

Clonality / Clone

This is a polyclonal antibody.

Host

ATG16 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Application

ATG16 antibody can be used for the detection of ATG16 by Western blot at 0.5 ?1 μg/ml.

Tested Application

E, WB, ICC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat.No. 4425P - ATG16 Peptide

Storage

ATG16 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

  1. Cat. No. 1201 - HeLa Cell Lysate

Species Reactivity

H, M, R

Protein GI Number

124256480

Protein Accession Number

NP_110430

Short Description

(NT) Autophagy related protein 16

This product belongs to the following categories:

  • Antibodies
  • Polyclonal Antibodies
  • Autophagy Antibodies

Related Products

  • APG7 Antibody
    (Catalog No. 3615).
  • ATG10 Antibody
    (Catalog No. 4399).
  • ATG12 Antibody
    (Catalog No. 4421).
  • ATG16 Antibody
    (Catalog No. 4427).
  • ATG16 Peptide
    (Catalog No. 4425P).
  • ATG5 Antibody
    (Catalog No. 4441).
  • TOR Antibody
    (Catalog No. 3485).

References

  1. Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.
  2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
  3. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
  4. Mizushima N, Noda T, and Ohsumi Y. Apg16p is required for the function of the apg12p-apg5p conjugate in the yeast autophagy pathway. EMBO J. 1999; 18:3888-96.


 

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