北京乐博生物科技有限公司是美国ProSci授权一级代理商，供应其全线产品涵盖了细胞凋亡、信号传导、肿瘤、免疫、细胞生物、神经生物和传染病等领域。产品应用于ELISA、WB、IHC、FACS等，为您的实验提供多样的检测手段，产品应用文献发表在PNAS、Cell research、Blood……使用广泛。

抗体信息如下：

Primary Antibodies

ATG12 Antibody

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.

Additional Names

ATG12 (NT), Autophagy protein 12, Autophagy related protein 12, APG12, APG12L, HAPG12

	Description Left: Western blot analysis of ATG12 in human brain tissue lysate with ATG12 antibody at (A) 0.5, and (B) 1 μg/ml. Below: Immunohistochemistry of ATG12 in human brain tissue with ATG12 antibody at 2.5 μg/ml.
	Other Product Images

Source

ATG12 antibody was raised against a 16 amino acid peptide from near the amino terminus of human ATG12.

Purification

Affinity chromatography purified via peptide column

Clonality / Clone

This is a polyclonal antibody.

Host

ATG12 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Application

ATG12 antibody can be used for the detection of ATG10 by Western blot at 0.5 ?1 μg/ml.

Tested Application

E, WB, IHC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat.No. 4421P - ATG12 Peptide

Storage

ATG12 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1303 - Human Brain Tissue Lysate

Species Reactivity

H, M, R

Protein GI Number

119569340

Protein Accession Number

EAW48955

Short Description

(NT) Autophagy protein 12

This product belongs to the following categories:

• Antibodies
• Polyclonal Antibodies
• Autophagy Antibodies

Related Products

• APG7 Antibody
  (Catalog No. 3615).
• ATG10 Antibody
  (Catalog No. 4399).
• ATG12 Antibody
  (Catalog No. 4423).
• ATG12 Peptide
  (Catalog No. 4421P).
• ATG5 Antibody
  (Catalog No. 4441).
• Brain Lysate
  (Catalog No. 1303).
• Brain Tissue Slide (Normal)
  (Catalog No. 10-301).
• RIG-1 Antibody
  (Catalog No. 3953).
• TOR Antibody
  (Catalog No. 3485).
• VISA Antibody
  (Catalog No. 4053).

References

1. Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.
2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
3. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
4. Hanada T, Noda NN, Satomi Y, et al. The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J. Biol. Chem. 2007; 282:37298-302.

更多现货抗体欢迎咨询乐博生物：010-59564854