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抗体信息如下：
Primary Antibodies
PIST Antibody
Background
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (4-6). Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1 (6).
Additional Names
PIST (CT), Golgi associated PDZ and coiled-coil motif containing / GOPC
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Description
Left: Western blot analysis of PIST in rat colon cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 μg/ml.
Below: Immunohistochemistry of PIST in rat colon tissue with PIST antibody at 1 μg/ml. |
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Other Product Images
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Source
PIST antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human PIST.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
PIST antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Virus PIST / Golgi associated PDZ and coiled-coil motif containing / GOPC (C-Terminus) Peptide (Cat. No. 3631P)
Application
PIST antibody can be used for the detection of PIST by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3631P - PIST Peptide
Storage
PIST antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1472 - Rat Colon Lysate
Species Reactivity
H, M, R
Protein GI Number
9837431
Protein Accession Number
AAG00572
Short Description
(CT) An autophagy related protein
This product belongs to the following categories:
- Antibodies
- Polyclonal Antibodies
- Apoptosis Antibodies
- Autophagy Antibodies
Related Products
- Autophagy Protein Detection Set
(Catalog No. PSI-1813).
- Beclin-1 Antibody
(Catalog No. 3611).
- Colon Lysate
(Catalog No. 1472).
- Multiple Species Tissue Array - Colon Tissue
(Catalog No. 10-700-MSTA).
- PIST Antibody
(Catalog No. 3633).
- PIST Antibody
(Catalog No. 46-194).
- PIST Peptide
(Catalog No. 3631P).
- TOR Antibody
(Catalog No. 3485).
References
- Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
- Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells.Carcinogenesis 1993; 14:2501-5.
- Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
- Yu Z, Horton A, Bravin M, et al. A novel protein complex linking the ?2 glutamate receptor and autophagy: implications for neurodegeneration in Lurcher mice. Neuron 2002; 35:921-33.
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