北京乐博生物科技有限公司是美国ProSci授权一级代理商，供应其全线产品涵盖了细胞凋亡、信号传导、肿瘤、免疫、细胞生物、神经生物和传染病等领域。产品应用于ELISA、WB、IHC、FACS等，为您的实验提供多样的检测手段，产品应用文献发表在PNAS、Cell research、Blood……使用广泛。

抗体信息如下：

Primary Antibodies

LAMP-2 Antibody

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). LAMP-2, a highly glycosylated protein associated with the lysosome (4), has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles (5) leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing Danon抯 disease is associated with the accumulation of autophagic material in striated myocytes (6). LAMP-2 exists in multiple isoforms (7).

Additional Names

LAMP-2, Lysosome associated membrane protein 2

	Description Left: Western blot analysis of LAMP-2 in HepG2 cell lysate with LAMP-2 antibody at (A) 1 and (B) 2 μg/ml. Below: Immunocytochemistry of LAMP-2 in HepG2 cells with LAMP-2 antibody at 10 μg/ml.
	Other Product Images

Source

LAMP-2 antibody was raised against a 17 amino acid peptide from near the carboxy of human LAMP-2.

Purification

Affinity chromatography purified via peptide column

Clonality / Clone

This is a polyclonal antibody.

Host

LAMP-2 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Human LAMP-2 Peptide (Cat. No. 3627P)

Application

LAMP-2 antibody can be used for the detection of LAMP-2 by Western blot at 1 ?2 μg/ml. (Optimal dilution should be determined by user). HepG2 cell lysate can be used as positive control. Despite its predicted size, LAMP-2 migrates at 115kDa in SDS-PAGE. LAMP-2 antibody is human and mouse reactive. This product is for research use only.

Tested Application

E, WB, ICC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat. No. 3627P - LAMP-2 Peptide

Storage

LAMP-2 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1211 - HepG2 Cell Lysate

Species Reactivity

H, M

Protein GI Number

7669503

Protein Accession Number

NP_054701

Short Description

An autophagy related protein

This product belongs to the following categories:

• Antibodies
• Polyclonal Antibodies
• Apoptosis Antibodies
• Autophagy Antibodies

Related Products

• Autophagy Protein Detection Set
  (Catalog No. PSI-1813).
• Hep G2 Cell Slide
  (Catalog No. 17-011).
• HepG2 Lysate
  (Catalog No. 1211).
• LAMP-1 Antibody
  (Catalog No. 3629).
• LAMP-2 Peptide
  (Catalog No. 3627P).
• TOR Antibody
  (Catalog No. 3485).

References

1. Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
3. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
4. Granger BL, Green SA, Gabel CA, et al. Characterization and cloning of the lgp110, a lysosomal glycoprotein from mouse and rat cells. J. Biol. Chem. 1990; 265:12036-43.

更多现货抗体欢迎咨询乐博生物：010-59564854