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抗体信息如下
Primary Antibodies
Beclin-1 Antibody
Background
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (3,4), is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors (5). Bcl-2 can bind to Beclin-1 and inhibit Beclin-1-dependent autophagy in yeast and mammalian cells, suggesting that Bcl-2 functions as an anti-autophagy protein as well as an anti-apoptotic protein, which helps maintain autophagy at levels that are more compatible with cell survival rather than cell death (6).
Additional Names
Beclin-1, Coiled-coil myosin-like Bcl-2-interacting protein
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Description
Left: Western blot analysis of Beclin-1 in 293 cell lysate with Beclin-1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
Below: Immunohistochemistry of beclin-1 in rat brain tissue with beclin-1 antibody at 2 μg/ml. |
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Other Product Images
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Source
Beclin-1 antibody was raised against a 17 amino acid peptide from near the amino terminus of human Beclin-1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Beclin-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Beclin-1 / Coiled-coil myosin-like Bcl-2-interacting protein (N-Terminus) Peptide (Cat. No. 3613P)
Application
Beclin-1 antibody can be used for the detection of Beclin-1 by Western blot at 0.5 - 1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3613P - Beclin-1 Peptide
Storage
Beclin-1 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1210 - 293 Cell Lysate
- Cat. No. 1403 - Mouse Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
16307457
Protein Accession Number
AAH10276
Short Description
An autophagy related protein
This product belongs to the following categories:
- Antibodies
- Polyclonal Antibodies
- Apoptosis Antibodies
- Autophagy Antibodies
- Cancer Antibodies
Related Products
- 293 Cell Slide
(Catalog No. 17-010).
- 293 Lysate
(Catalog No. 1210).
- APG7 Antibody
(Catalog No. 3615).
- Bcl-2 Antibody
(Catalog No. 3335).
- Beclin-1 Antibody
(Catalog No. 3611).
- Beclin-1 Peptide
(Catalog No. 3613P).
- Brain Lysate
(Catalog No. 1403).
- Multiple Species Tissue Array - Brain Tissue
(Catalog No. 10-300-MSTA).
References
- Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
- Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
- Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J. Virol. 1998; 72:8586-96.
- Kametaka S, Okano T, Ohsumi M, et al. Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 1998; 273:22284-91.
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