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抗体信息如下：
Primary Antibodies
PDL-1 Antibody
Background
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. PDL-1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. This suggests that PDL-1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts. At least two isoforms of PDL-1 are known to exist; this antibody is specific to the larger isoform. PDL-1 antibody has no cross-reactivity to PDL-2.
Additional Names
PDL-1, Programmed cell death 1 ligand-1, programmed death ligand 1, PD-L1, B7-H1
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Description
Left: Western blot analysis of PDL-1 in Raji cell lysate with PDL-1 antibody at (A) 0.5 and (B) 1 μg/ml.
Below: Immunohistochemistry of PDL-1 in human heart tissue with PDL-1 antibody at 2.5 μg/ml. |
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Other Product Images
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Source
PDL-1 antibody was raised against a 17 amino acid peptide from near the center of human PDL-1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
PDL-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
PDL-1 antibody can be used for detection of PDL-1 by Western blot at 0.5 ?1 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4059P - PDL-1 Peptide
Storage
PDL-1 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1207 - Raji Cell Lysate
- Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
7661534
Protein Accession Number
NP_054862
Short Description
A novel ligand of PDCD-1
This product belongs to the following categories:
- Antibodies
- Polyclonal Antibodies
- Apoptosis Antibodies
Related Products
- Heart Lysate
(Catalog No. 1301).
- Heart Tissue Slide (Normal)
(Catalog No. 10-501).
- PD-1 Antibody
(Catalog No. 4065).
- PD-1 Antibody
(Catalog No. 4067).
- PD-L1 Antibody
(Catalog No. 46-156).
- PDL-1 Peptide
(Catalog No. 4059P).
- PDL-2 Antibody
(Catalog No. 4063).
- Raji Cell Slide
(Catalog No. 17-007).
- Raji Lysate
(Catalog No. 1207).
References
- Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
- Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
- LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78.
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