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抗体信息如下：

Primary Antibodies

EndoG Antibody

Background

The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).

Additional Names

EndoG

	Description Left: Western blot analysis of EndoG in mouse (M) 3T3 and human (H) HepG2 cell lysates with EndoG antibody at 2 μg/ml. Below: Immunohistochemistry of EndoG in human pancreas with EndoG antibody at 15 μg/ml.
	Other Product Images

Source

EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.

Purification

Affinity chromatography purified via peptide column

Clonality / Clone

This is a polyclonal antibody.

Host

EndoG antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Human EndoG Peptide (Cat. No. 3035P)

Application

EndoG antibody can be used for detection of EndoG by Western blot at 1 to 2 μg/ml. HepG2 and 3T3 cell lysates can be used as positive control and a 35 kDa band can be detected. It is human, mouse and rat reactive.

Tested Application

E, WB, IHC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat. No. 3035P - EndoG Peptide

Storage

EndoG antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1211 - HepG2 Cell Lysate
2. Cat. No. 1212 - 3T3 Whole Cell Lysate
3. Cat. No. 1307 - Human Pancreas Tissue Lysate

Species Reactivity

H, M, R

Protein GI Number

53759134

Protein Accession Number

NP_004426

Short Description

Endonuclease G

This product belongs to the following categories:

• Antibodies
• Polyclonal Antibodies
• Apoptosis Antibodies
• Cancer Antibodies

Related Products

• 3T3 (Balb) Lysate
  (Catalog No. 1212).
• 3T3/NIH Cell Slide
  (Catalog No. 17-202).
• EndoG Fragment Recombinant Protein
  (Catalog No. 95-103).
• EndoG Monoclonal Antibody
  (Catalog No. PM-4581).
• EndoG Monoclonal Antibody
  (Catalog No. PM-4583).
• EndoG Monoclonal Antibody
  (Catalog No. PM-4577).
• EndoG Monoclonal Antibody
  (Catalog No. PM-4579).
• EndoG Peptide
  (Catalog No. 3035P).
• Hep G2 Cell Slide
  (Catalog No. 17-011).
• HepG2 Lysate
  (Catalog No. 1211).
• Pancreas Lysate
  (Catalog No. 1307).
• Pancreas Tissue Slide (Normal)
  (Catalog No. 11-101).

References

1. Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature. 2001;412(6842):95-9.
2. Parrish J, Li L, Klotz K, Ledwich D, Wang X, Xue D. Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature. 2001;412(6842):90-4
3. Hengartner MO. Apoptosis. DNA destroyers. Nature. 2001;412(6842):27, 29.
4. Widlak P, Li LY, Wang X, Garrard WT. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J Biol Chem. 2001;276(51):48404-9.

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