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抗体信息如下：

Primary Antibodies

AIF Antibody

Background

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli (2,3). AIF is highly conserved between human and mouse and widely expressed (2).

Additional Names

AIF (IN), AIF

	Description Left: Western blot analysis of AIF in K562 cell lysate (A), rat heart (B), and mouse heart (C) tissue lysates with AIF antibody (IN) at 1 μg/ml. Below: Immunohistochemistry of AIF in human retina with AIF antibody at 10 μg/ml.
	Other Product Images

Source

AIF antibody was raised against a peptide corresponding to amino acids 517 to 531 of human AIF. This sequence is identical to those of mouse and rat AIF.

Purification

Ion exchange chromatography purified

Clonality / Clone

This is a polyclonal antibody.

Host

AIF antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Human AIF (Intermediate Domain) Peptide (Cat. No. 2267P)

Application

AIF antibody can be used for detection of AIF by Western blot at 0.25 to 1 μg/ml. A 67 kDa band should be detected.

Tested Application

E, WB, IHC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat. No. 2267P - AIF Peptide

Storage

AIF antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1204 - K562 Cell Lysate
2. Cat. No. 1461 - Rat Heart Tissue Lysate
3. Cat. No. 1401 - Mouse Heart Tissue Lysate

Species Reactivity

H, M, R

Protein GI Number

13431764

Protein Accession Number

O95831

Short Description

(IN) Apoptosis Inducing Factor

This product belongs to the following categories:

• Antibodies
• Polyclonal Antibodies
• Apoptosis Antibodies

Related Products

• AIF Peptide
  (Catalog No. 2267P).
• Heart Lysate
  (Catalog No. 1401).
• Heart Lysate
  (Catalog No. 1461).
• K-562 Cell Slide
  (Catalog No. 17-004).
• K562 Lysate
  (Catalog No. 1204).
• Multiple Species Tissue Array - Heart Tissue
  (Catalog No. 10-500-MSTA).

References

1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8
2. Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999;397:441-6
3. Daugas E, Susin SA, Zamzami N, et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J 2000;14:729-39 (WD0500)

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