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抗体信息如下：

Additional Names

Apaf1 (NT), Apaf1, Apaf-1

	Description Left: Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at 1 μg/ml in the (A) absence and (B) presence of blocking peptide. Below: Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/ml.
	Other Product Images

Source

Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.

Purification

Affinity chromatography purified via peptide column

Clonality / Clone

This is a polyclonal antibody.

Host

Apaf1 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Human Apaf1 (N-Terminus) Peptide (Cat. No. 2013P)

Application

Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 ?2 μg/ml. (Optimal dilution should be determined by user.) Whole cell lysate from HeLa cells can be used as positive control and a 115 - 130 kDa band should be detected.

Tested Application

E, WB, IHC

Buffer

Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide

Cat. No. 2013P - Apaf1 Peptide

Storage

Apaf1 antibody can be stored at 4?C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control

1. Cat. No. 1204 - K562 Cell Lysate

Species Reactivity

H, M, R

Protein GI Number

2330015

Protein Accession Number

AAC51678

Short Description

(NT) Apoptotic protease activating factor 1

This product belongs to the following categories:

• Antibodies
• Polyclonal Antibodies
• Apoptosis Antibodies
• Cancer Antibodies

Related Products

• Apaf-1 Monoclonal Antibody
  (Catalog No. PM-2015).
• Apaf-1 Monoclonal Antibody
  (Catalog No. PM-2016).
• APAF1 Antibody
  (Catalog No. XW-7034).
• Apaf1 Peptide
  (Catalog No. 2013P).
• K-562 Cell Slide
  (Catalog No. 17-004).
• K562 Lysate
  (Catalog No. 1204).

References

1. Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13
2. Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998;94:727-37
3. Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation ofApaf1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89
4. Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts with Apaf1 and inhibits Apaf1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998;95:4386-91 (RD1299)