PhosSTOP tablets are a proprietary blend of phosphatase inhibitors,
formulated as a ready-to-use tablet which dissolves quickly in aqueous
solutions (buffers), including buffers containing formalin (for formalin
fixation).
Working concentration: 1 tablet per 10 ml cell extract or buffer
Working solution: Solvent is recommended in distilled water or aqueous buffers (including buffers containing formalin).
Dissolve one tablet per 10 ml aqueous buffer. Alternatively, prepare a 10x stock solution in 1 ml dist. water or 100 mM phosphate buffer, pH 7.0. Higher concentrations of stock solutions (e.g., 20x) are possible.
Storage conditions (working solution): 2 to 8 °C
Specificity
Protects proteins against dephosphorylation. Inhibits phosphatase classes such as acid and alkaline phosphatases, as well as serine/threonine (PP1, PP2A, and PP2B) and tyrosine protein phosphatases (PTP) in bacterial, mammalian, yeast, and plant extracts.
Application
PhosSTOP? protects protein extracts against dephosphorylation. The tablets inhibit a broad spectrum of phosphatases such as acidic and alkaline phosphatases, as well as serine/threonine (PP1, PP2A, and PP2B), dual-specificity phosphatases and tyrosine protein phosphatases (PTP) in bacterial, insect, mammalian, yeast, and plant extracts. PhosSTOP Tablets are well suited for the purification and detection of phoshorylated proteins.They are also used to prevent dephosphorylation in formalin-fixed paraffin embedded (FFPE) tissue sections.
PhosSTOP tablets can be applied in combination with cOmplete Protease Inhibitor Cocktail Tablets to simultaneously protect proteins against dephosphorylation and proteolytic degradation. Both inhibitor tablets maintain their activity when used together.
PhosSTOP inhibitors were evaluated for their effect on protein assays. The inhibition shows almost no influence on BCA or Bradford protein assays.