产品描述
cOmplete, EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum
of serine and cysteine proteases. In contrast to other cOmplete tablets
they do not contain EDTA - no other chelating agents, e.g. EGTA
as well - leaving the stability and the function of metal-dependant
proteins unaffected. The affinity purification of Poly-His-tagged fusion
proteins using IMAC (Immobilized Metal Affinity Chromatography) is also
facilitated (no dialysis necessary).
Due to the optimized
composition of the tablets, they show excellent inhibition of serine and
cysteine proteases, and are well suited for the protection of proteins
isolated from animal tissues, plants, yeast, and bacteria. cOmplete,
EDTA-free tablets, contains both irreversible and reversible protease
inhibitors. Metallo- and aspartic proteases are not inhibited.
cOmplete EDTA-free Tablets are identical to cOmplete Tablets, the only difference being that no EDTA or other chelating (e.g. EGTA) agents are included.
Working concentration: 1 tablet per 50 ml extraction solution
Working solution: Handling
Carefully
push the tablet through the foil packaging using the base of your thumb
(not fingernail) to prevent the breakage of tablets.
Preparation of Working Solution
One
cOmplete EDTA-free tablet is sufficient for the inhibition of the
proteolytic activity in 50 ml extraction solution. When very high
proteolytic activity is present, one tablet should be used for 25 ml
extraction buffer. Tablets can be added directly to the extraction
medium. Alternatively, a stock solution (25 X conc.) can be prepared.
Preparation of Stock solution (25 X conc.)
Dissolve one cOmplete EDTA-free tablet in 2 ml dist. water or in 2 ml 100 mM phosphate buffer, pH 7.0.
Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.