The monoclonal anti-Taq DNA Polymerase antibodies were derived from a hybridoma cell line. Anti-Taq antibodies are mouse IgG type. The storage buffer is 10 mM Tris · HCl (pH 7.0 at 22°C); 50 mM KCl; 0.1 mM EDTA; 50 % glycerol.
Associated activities: No conversion to the covalently closed circular DNA to the nicked or linear form was observed after incubation of 1 μg of pUC19 DNA with antibodies in a final concentration of 2.5 mg/ml in 20 μl of reaction mixture containing 25 mM Tris · HCl (pH 7.9); 100 mM NaCl; 10 mM MgCl2 after 16 hours at 37°C.
Unit definition: One unit of activity is defined as the amount of antibody required to block 50 % of the activity of 1 μg Taq DNA polymerase at 37°C.