(1) Needles, H.L. (1970) Anal. Biochem. 35, 533-537
Effect of solution components on large-pore polyacrylamide gel formation.
(2) Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87
Electrophoresis in agarose and acrylamide gels.
(3) Gomes, A.V. & Barnes, J.A. (1998) Anal. Biochem. 260, 106-108
Gel electrophoresis of mini gels.
TEMED was introduced as an enhancer of the polymersitation (cross-linking) of acrylamide and bisacrylamide in gel electrophoresis. It catalysis the formation of free radicals of the initiator of the polymerisation, ammonium persulfate. If gels are degased to remove oxygen, add the TEMED after degasing. The working concentration is 50 μl / 100 ml of gel solution. TEMED is stable (< two years), if it is protected from taking up water.
Caution: TEMED is harmful. Handle under a chemical fume hood. Do not breathe fumes!