(1) Ogden, R.C. & Adams, D.A. (1987) Methods Enzymol. 152, 61-87
Electrophoresis in agarose and acrylamide gels.
(2) Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edition; Page 6.36. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
(3) Ausubel, F.A., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K. (eds.) (1995) Current Protocols in Molecular Biology. Greene Publishing & Wiley-Interscience, New York.
Polyacrylamide gels are formed by the polymerization of acrylamide and the cross-linking by a bifunctional agent, usually N,N''''-Methylenebisacrylamide. The porosity of the gel is determined by the concentration of acrylamide and the ratio of acrylamide : cross-linker.
Bisacrylamide is more hydrophobic than acrylamide, but can be dissolved in lukewarm water. Like acrylamide all solutions of bisacrylamide should be filtered.