Other Notes View more information on collagenase and cell dissociation at www.sigma-aldrich.com/enzymeexplorer.
Preparation Note Equivalent to first 40% ammonium sulfate fraction of Mandl, I., et al., J. Clin. Invest., 32, 1323 (1953).
Quality Also contains clostripain, nonspecific neutral protease, and tryptic activities.
Unit Definition One collagen digestion unit liberates peptides from collagen equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hr at pH 7.4 at 37 °C in the presence of calcium ions.
One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions.
One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37 °C.
One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25 °C in the presence of DTT.
Properties
suitability release of physiologically active rat hepatocytes tested
storage temp. -20°C
现货供应，原装1g包装，有100mg包装！
报价：2400元/1g 640元/100mg