Remote control and non-rotating plunger eliminates vibration allowing precise injection minimizing possible cell rupture
Selectable Injection Volumes: from 2.3 nanoliters to 69.0 nanoliters in 16 increments
Selectable Operating Speeds:
Fill Speed
Fast
46 nL per second
Slow
23 nL per second
Injection Speed
Fast
46 nL per second
Slow
23 nL per second
Empty Speed
Fast
230 nL per second
Slow
92 nL per second
Supplied with sufficient borosilicate glass capillaries for preparing 400 injection tips, standard softening point 780°C
Can be mounted on most micromanipulators Note: Universal Adapter 3-000-024-A supplied with unit
Separate Foot Switch available to allow hands-free operation
Each Nanoject II Auto-Nanoliter Injector comes complete with 200 borosilicate capillaries that can be pulled to the required tolerance together with a back filling needle and maintenance kit.
Catalog Information
Cat. No.
Description
3-000-204
Nanoject II Variable Volume (2.3 to 69 nL) Automatic Injector with Glass Capillaries (110V)
3-000-205A
Nanoject II, 220V, CE, Euro
3-000-206A
Nanoject II, 220V, CE, UK
Note: Universal Adapter supplied with all the above units
Replacement Parts
Cat. No.
Description
3-000-002-KN
O-Ring kit for Models 204, 205A, and 206A includes: 1 collet, 1 small O-Ring, 1 spacer, 1 compression O-Ring, and 1 Allen wrench
3-000-027
30 gauge, 2" Backfilling Needle
3-000-000-203-X
Pointed Plunger Wire
Replacement Glass Capillaries
Cat. No.
O.D (mm)
Length
I.D. (mm)
Pack
3-000-203-G/X
1.14
3.5"
0.53
100
3-000-203-G/XL
1.14
7"
0.53
100
This equipment is ready for use as received. It does require pulled capillary tips. It is essential that only the capillaries supplied or exact replacement capillaries are used for pulling micropipets.
Contents:
a. Injector head with cord.
b. Control box.
c. Power supply with cord.
d. Capillary glass; 2 vials of 100 pieces per vial:
e. Replacement O-ring kit with collet, standard. (Cat. No. 3-000-002-KN)
f. 1 Allen wrench for replacing wire plunger.
g. 1 30g x 2" needle for backfilling pipets. Cat. No. 3-000-027
Operation:
The injector head should be mounted in a suitable micromanipulator or stereotaxic unit. Marzhauser model MM33 is quite suitable and frequently used. The aluminum barrel with the Drummond logo, provides an ideal clamping surface. Its diameter measures 11mm.The precise operation of the Nanoject II depends greatly on the use of tips prepared from the glass provided. We cannot guarantee that glass micropipets pulled from capillaries with other dimensions will work. Never attempt to use micropipets pulled from filament glass. Damage to the wire plunger will result and injection volumes will not be accurate.
Micropipet Pulling and Backfilling
The Nanoject II requires the use of pulled micropipets from the glass provided. Ideally, the tip size should be 10-30 microns in size. The capillary glass provided is produced from N-51-A material and has a softening point of 780o C. Many researchers pull the tips and then break them off with forcepts. This enables piercing the cell membrane much easier with no skipping. Once the tips are pulled, they must be "backfilled" with oil (or other non-compressible fluid) before attachment to the injector. Silicone or mineral oil is frequently used. Backfilling is facilitated by using the 30g x 2" needle and a syringe. Disposable spinal needles are also frequently used.
NOTE: THE NANOJECT II WILL NOT OPERATE PROPERLY WITHOUT BACKFILLING THE MICROPIPET.
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