产品介绍
INTENDED USE
The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IFNa in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
SENSITIVITY
The minimum detectable dose of IFNa is typically less than 3.0pg/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
SPECIFICITY
This assay has high sensitivity and excellent specificity for detection of IFNa.
No significant cross-reactivity or interference between IFNa and analogues was observed.
Note:
Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between IFNa and all analogues, therefore, cross reactivity may still exist.
PRECISION
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IFNa were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IFNa were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
STABILITY
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Note:
To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
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