产品介绍
小鼠脂联素(ADP) ELISA Kit DL-ADP-Mu试剂盒内容：
96孔板(预包被) | 1 | 96孔板覆膜 | 2 |
标准品 | 2 | 标准品稀释液 | 1×20mL |
检测溶液A | 1×120μL | 检测稀释液A | 1×12mL |
检测溶液B | 1×120μL | 检测稀释液B | 1×12mL |
TMB底物 | 1×9mL | 终止液 | 1×6mL |
浓洗涤液(30×) | 1×20mL | 使用说明书 | 1 |
SENSITIVITY
The minimum detectable dose of ADP is typically less than 14.5pg/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
SPECIFICITY
This assay has high sensitivity and excellent specificity for detection of ADP.
No significant cross-reactivity or interference between ADP and analogues was observed.
Note:
Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between ADP and all analogues, therefore, cross reactivity may still exist.
PRECISION
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ADP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ADP were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
STABILITY
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.
Note:
To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
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