来宝网移动站

咨询电话：15204658329。联系人：裴先生。销售商：哈尔滨哈逻生物科技有限公司

Application:  PCR purification

Downstream Application Techniques: PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger and Next Generation), cloning, primer walking

Agencourt AMPure XP Features

• High recovery of amplicons, greater than 100 bp
• Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
• Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days
• Efficient recovery of double stranded and single stranded DNA templates
• Consistent recovery throughout the kit’s 12 month shelf life
• Faster manual and automated processing as compared to traditional post-PCR cleanup methods

Process Overview

1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate

High Recovery

Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods (Figure 1).

Figure 1. The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products where purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system.

Walk-away Automated Solution

Agencourt AMPure XP post-PCR cleanup has been automated on three Beckman Coulter liquid handlers, the Biomek 3000, NXP and FXP for processing scalability. The LIMS compatible automation functionality is aided by a user-friendly interface equipped with a quick startup menu, a deck setup walk-through menu, a method recovery menu, and a procedure menu.

Ordering Information

To request a quote or make an inquiry use the on-line contact form. In the United States and Canada call +1 800 361 7780. Outside the United States and Canada consult the International Distributors list.