4-Methylumbelliferyl beta-D-glucuronide (MUG) is a fluorogenic substrate for beta-glucuronidase (GUS). Upon cleavage, MUG yields blue fluorescence seen under UV light.
This substrate is commonly used for the detection of Escherichia coli through its ability to detect activity of beta-glucuronidase, which is encoded by uidA. GUS activity is tested in minimal medium o-nitrophenyl-beta-D-galactopyranoside (MMO)-MUG.
It is ideal for plant molecular study as a way to examine gene expression under various promoters. And while it works well for measuring activity in plant lysate and leaf disks, it can also work in whole plant assays.
Product Specifications
MUG
4-Methylumbelliferyl beta-D-glucuronide
MOLECULAR BIOLOGY GRADE
Formula: C 16H16O9
MW: 352.3 g/mol
Storage/Handling: Store Desiccated at -20°C
PubChem Chemical ID: 3683393
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