产品介绍
iSWAB RNA Collection Kit
(ISWAB-RNA-1200)
Collect-Stabilize-Concentrate-Transport-Extract-Store All in a Single Tube:
The iSWAB-RNA device allows for the collection, concentration, and stabilization of RNA at the point of collection. Maintaining RNA stability and protecting it from degradation is a significant challenge, and current RNA stabilization methods such as PAXgene, Tempus, RNAgard or dry blood spots require invasive blood collection resulting in low compliance. The need to collect blood results in a very limited shelf life, forcing labs to invest in cold storage infrastructure or commit resources to immediate sample processing. Other options for non-invasive collection also suffer from short shelf life storage as well as low yields.
Features and Benefits:
Increase process efficiency: Extended shelf life stability (6 months), reduced processing time, and minimal storage footprint
Swab-free sample transport: Decrease sample processing time without compromising sample integrity
Up to 6μg of Total RNA: High quality RNA for downstream applications such as RT-PCR, Microarray and RNA-seq for gene expression analysis
Achieve less than 1% bacterial genomic DNA contamination: Enables accurate and reliable downstream analysis
Room temperature stable: Reduce sample storage and transport costs by eliminating cold chain requirements
Self collection or assisted collection in less than 5 minutes: Suitable for all population segments including infants, toddlers, and elderly
Traceable and reliable chain of custody: LIMS compatible unique bar-codes included on each collection device for efficient traceability and storage purposes
Scalable and easy to process: Manual and automation friendly sample processing
Superior Performance over Non-Invasive and Invasive Methods
Comparison of Average RNA Yields Isolated from Different Collection Methods
| Non-Invasive | Invasive (Whole Blood) |
| iSWAB-RNA | Competitor O-R | Competitor Pg | Competitor Ts | Competitor Rg |
| Avg. Yield (μg/mL) | 4.0 | 0.2 | 1.3 | 0.6 | 2.0 |
| STDEV | 1.2 | 0.17 | 0.59 | 0.42 | 0.71 |
All samples (n=12) were processed with QIAamp RNA Blood Mini Kit. The RNA concentration was measured with Nanodrop and Quant-iT? RiboGreen? RNA Reagent
High Quality RNA
Comparison of Average OD Readings of Samples from Different Collection Methods
| PRODUCT | OD 260/280 | OD 260/230 | RIN |
| iSWAB-RNA | 1.97 ± 0.0.13 | 2.1 ± 0.21 | 8.9 ± 0.3 |
| Competitor O-R | 1.66 ± 0.0.23 | 1.48 ± 0.61 | 7.1 ± 0.5 |
| Competitor Pg | 2.03 ± 0.0.08 | 1.56 ± 0.31 | 8.1 ± 0.2 |
| Competitor Ts | 1.83 ± 0.4 | 2.01 ± 0.14 | 8.0 ± 0.2 |
| Competitor Rg | 1.9 ± 0.15 | 2.0 ± 0.8 | 8.4 ± 0.5 |
All samples (n=12) were measured using a Nanodrop
来宝会员