产品介绍
The iSWAB? collection devices have been designed to address the low DNA recovery and quality (ie. high content of bacterial DNA), cost and processing issues that current noninvasive sample collection approaches suffers from. The innovative design of iSWAB? collection devices allow for removal of the cells captured by the swab and then released into a proprietary lysis and nucleic acid and protein stabilizing buffer. The combination of the mechanistic release of cells and proprietary lysis buffer allows for the collection of high amount of cells in a concentrated manner from single or several swabs in a small amount of lysis buffer while marinating significantly low bacterial contamination. iSWAB? collection devices are designed to facilitate self or assisted sample collection, sample concentration, transport, extraction and for long term storage at room temperature, all in a single tube. In addition the iSWAB? collection device is optimized for manual processing as well as it can be adapted to common automated sample preparation liquid handling workstations without the need of additional accessories.
iSWAB RNA Collection Kit
(ISWAB-RNA-1200)
Collect-Stabilize-Concentrate-Transport-Extract-Store All in a Single Tube:
The iSWAB-RNA device allows for the collection, concentration, and stabilization of RNA at the point of collection. Maintaining RNA stability and protecting it from degradation is a significant challenge, and current RNA stabilization methods such as PAXgene, Tempus, RNAgard or dry blood spots require invasive blood collection resulting in low compliance. The need to collect blood results in a very limited shelf life, forcing labs to invest in cold storage infrastructure or commit resources to immediate sample processing. Other options for non-invasive collection also suffer from short shelf life storage as well as low yields.
Features and Benefits:
Increase process efficiency: Extended shelf life stability (6 months), reduced processing time, and minimal storage footprint
Swab-free sample transport: Decrease sample processing time without compromising sample integrity
Up to 6μg of Total RNA: High quality RNA for downstream applications such as RT-PCR, Microarray and RNA-seq for gene expression analysis
Achieve less than 1% bacterial genomic DNA contamination: Enables accurate and reliable downstream analysis
Room temperature stable: Reduce sample storage and transport costs by eliminating cold chain requirements
Self collection or assisted collection in less than 5 minutes: Suitable for all population segments including infants, toddlers, and elderly
Traceable and reliable chain of custody: LIMS compatible unique bar-codes included on each collection device for efficient traceability and storage purposes
Scalable and easy to process: Manual and automation friendly sample processing
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Performance
Literature
Application Notes
Superior Performance over Non-Invasive and Invasive Methods
Comparison of Average RNA Yields Isolated from Different Collection Methods
| Non-Invasive | Invasive (Whole Blood) |
| iSWAB-RNA | Competitor O-R | Competitor Pg | Competitor Ts | Competitor Rg |
| Avg. Yield (μg/mL) | 4.0 | 0.2 | 1.3 | 0.6 | 2.0 |
| STDEV | 1.2 | 0.17 | 0.59 | 0.42 | 0.71 |
All samples (n=12) were processed with QIAamp RNA Blood Mini Kit. The RNA concentration was measured with Nanodrop and Quant-iT? RiboGreen? RNA Reagent
High Quality RNA
Comparison of Average OD Readings of Samples from Different Collection Methods
| PRODUCT | OD 260/280 | OD 260/230 | RIN |
| iSWAB-RNA | 1.97 ± 0.0.13 | 2.1 ± 0.21 | 8.9 ± 0.3 |
| Competitor O-R | 1.66 ± 0.0.23 | 1.48 ± 0.61 | 7.1 ± 0.5 |
| Competitor Pg | 2.03 ± 0.0.08 | 1.56 ± 0.31 | 8.1 ± 0.2 |
| Competitor Ts | 1.83 ± 0.4 | 2.01 ± 0.14 | 8.0 ± 0.2 |
| Competitor Rg | 1.9 ± 0.15 | 2.0 ± 0.8 | 8.4 ± 0.5 |
All samples (n=12) were measured using a Nanodrop
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